Mammalian Genome 9, 40810 (1998). Mymmafian Genome Incorporating Mouse Censure © Springer-Verlag New York Inc. 1998 Li-Yan Yin, Yaxu Wu, Carol A. Ballinger, Cam Patterson Division of Cardiology, University of Texas Medical Branch at Galveston, 301 University Boulevard, 9.138 Medical Research Building, Galveston, Texas 77555-1064, USA Received: 9 September 1997 / Accepted: 29 December 1997 Signaling by members of the receptor tyrosine kinase (RTK) gene Genomic organization of the human KDR/flk-1 gene. Our previ- ous data indicated that the KDR/flk-1 gene spanned a large seg- family is critically important in differentiation and growth of many, if not all, cell types (Ullrich and Schlessinger 1990). Vas- ment of the genome (Patterson et al. 1995). Therefore, we elected cular endothelial cells in particular are dependent on pathways to isolate P1 plasmids to facilitate the cloning and mapping of this mediated by RTKs for normal embryological development and gene. A human genomic DNA bacteriophage P1 library (Genome Systems, Inc., St. Louis, Mo.) was screened by polymerase chain proliferation. In addition to expressing non-lineage restricted reaction (PCR) with primers based on the published human KDR/ RTKs such as receptors for fibroblast growth factors, epidermal growth factor, and the platelet-derived growth factors, vascular flk-1
Mammalian Genome – Springer Journals
Published: Mar 27, 2009
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