Genome-wide screening differential long non-coding RNAs expression profiles discloses its roles involved in OHSS development

Genome-wide screening differential long non-coding RNAs expression profiles discloses its roles... Objective To screen differentially expressed lncRNAs involved in OHSS. OHSS is defined as ovarian hyperstimulation syn- drome. It is characterized as enlarged ovary and increased vascular permeability. Design Case-control study. Setting University-affiliated hospital. Patient(s) Patients with OHSS high risk (n = 30) and low risk (n = 30) were included in this study. Intervention(s) None. Main outcome measure(s) LncRNAs from women with OHSS high risk and low risk were used for high-throughput sequencing profiling. The eight most differentially expressed lncRNAs in granulosa cells were validated by semi-quantitative reverse transcription-polymerase chain reaction analysis. Result(s) A total of 23,815 lncRNAs were detected and 482 were differentially expressed (fold-change ≥2; p <0.05, FDR value < 0.001), of which 205 were upregulated and 277 were downregulated. Lnc-SEC16B.1-6, lnc-SNURF-13, lnc-LGR6-6, and lnc- H2AFY2-2 were up-regulated, while lnc-BRD2-2, lnc-HSPA6-2, and lnc-CLIC6-5 were downregulated significantly in granu- losa cells. These results were confirmed by qRT-PCR. KEGG pathways and Gene Ontology enrichment analysis revealed that several biological processes were significantly associated. Meanwhile, the lncRNA/miRNA interaction network was established according to ceRNA network model. Conclusion(s) Comprehensive expression screening identified eight novel lncRNAs associated with risk factors of OHSS pro- cess. Although it is unclear how these altered lncRNAs http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Assisted Reproduction and Genetics Springer Journals

Genome-wide screening differential long non-coding RNAs expression profiles discloses its roles involved in OHSS development

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Publisher
Springer US
Copyright
Copyright © 2018 by Springer Science+Business Media, LLC, part of Springer Nature
Subject
Medicine & Public Health; Gynecology; Reproductive Medicine; Human Genetics
ISSN
1058-0468
eISSN
1573-7330
D.O.I.
10.1007/s10815-018-1199-0
Publisher site
See Article on Publisher Site

Abstract

Objective To screen differentially expressed lncRNAs involved in OHSS. OHSS is defined as ovarian hyperstimulation syn- drome. It is characterized as enlarged ovary and increased vascular permeability. Design Case-control study. Setting University-affiliated hospital. Patient(s) Patients with OHSS high risk (n = 30) and low risk (n = 30) were included in this study. Intervention(s) None. Main outcome measure(s) LncRNAs from women with OHSS high risk and low risk were used for high-throughput sequencing profiling. The eight most differentially expressed lncRNAs in granulosa cells were validated by semi-quantitative reverse transcription-polymerase chain reaction analysis. Result(s) A total of 23,815 lncRNAs were detected and 482 were differentially expressed (fold-change ≥2; p <0.05, FDR value < 0.001), of which 205 were upregulated and 277 were downregulated. Lnc-SEC16B.1-6, lnc-SNURF-13, lnc-LGR6-6, and lnc- H2AFY2-2 were up-regulated, while lnc-BRD2-2, lnc-HSPA6-2, and lnc-CLIC6-5 were downregulated significantly in granu- losa cells. These results were confirmed by qRT-PCR. KEGG pathways and Gene Ontology enrichment analysis revealed that several biological processes were significantly associated. Meanwhile, the lncRNA/miRNA interaction network was established according to ceRNA network model. Conclusion(s) Comprehensive expression screening identified eight novel lncRNAs associated with risk factors of OHSS pro- cess. Although it is unclear how these altered lncRNAs

Journal

Journal of Assisted Reproduction and GeneticsSpringer Journals

Published: Jun 4, 2018

References

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