Objective To screen differentially expressed lncRNAs involved in OHSS. OHSS is defined as ovarian hyperstimulation syn- drome. It is characterized as enlarged ovary and increased vascular permeability. Design Case-control study. Setting University-affiliated hospital. Patient(s) Patients with OHSS high risk (n = 30) and low risk (n = 30) were included in this study. Intervention(s) None. Main outcome measure(s) LncRNAs from women with OHSS high risk and low risk were used for high-throughput sequencing profiling. The eight most differentially expressed lncRNAs in granulosa cells were validated by semi-quantitative reverse transcription-polymerase chain reaction analysis. Result(s) A total of 23,815 lncRNAs were detected and 482 were differentially expressed (fold-change ≥2; p <0.05, FDR value < 0.001), of which 205 were upregulated and 277 were downregulated. Lnc-SEC16B.1-6, lnc-SNURF-13, lnc-LGR6-6, and lnc- H2AFY2-2 were up-regulated, while lnc-BRD2-2, lnc-HSPA6-2, and lnc-CLIC6-5 were downregulated significantly in granu- losa cells. These results were confirmed by qRT-PCR. KEGG pathways and Gene Ontology enrichment analysis revealed that several biological processes were significantly associated. Meanwhile, the lncRNA/miRNA interaction network was established according to ceRNA network model. Conclusion(s) Comprehensive expression screening identified eight novel lncRNAs associated with risk factors of OHSS pro- cess. Although it is unclear how these altered lncRNAs
Journal of Assisted Reproduction and Genetics – Springer Journals
Published: Jun 4, 2018
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