Genome sequence of a novel endornavirus from the phytopathogenic fungus Alternaria brassicicola

Genome sequence of a novel endornavirus from the phytopathogenic fungus Alternaria brassicicola In an effort to discover new mycoviruses from phytopathogenic fungi, a dsRNA molecule of 10,290 nt, resembling those associated with the viruses belonging to the family Endornaviridae , was isolated from Alternaria brassicicola , one of the causal agents of rapeseed black spot disease. Genome analysis revealed the presence of a single open reading frame coding for a polyprotein of 3400 aa containing conserved viral methyltransferase (MTR), viral RNA helicase 1 (Hel-1), and RNA-dependent RNA polymerase (RdRp) domains. In addition, a cysteine-rich region (CRR) with conserved CXCC motifs, shared among several endornaviruses, was also identified between the MTR and Hel-1 domains. Phylogenetic analysis based on the RdRp sequence strongly suggested that the virus infecting A. brassicicola should be considered a representative of a novel endornavirus species, and this virus was designated as Alternaria brassicicola endornavirus 1 (AbEV1). http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Genome sequence of a novel endornavirus from the phytopathogenic fungus Alternaria brassicicola

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Publisher
Springer Vienna
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2426-y
Publisher site
See Article on Publisher Site

Abstract

In an effort to discover new mycoviruses from phytopathogenic fungi, a dsRNA molecule of 10,290 nt, resembling those associated with the viruses belonging to the family Endornaviridae , was isolated from Alternaria brassicicola , one of the causal agents of rapeseed black spot disease. Genome analysis revealed the presence of a single open reading frame coding for a polyprotein of 3400 aa containing conserved viral methyltransferase (MTR), viral RNA helicase 1 (Hel-1), and RNA-dependent RNA polymerase (RdRp) domains. In addition, a cysteine-rich region (CRR) with conserved CXCC motifs, shared among several endornaviruses, was also identified between the MTR and Hel-1 domains. Phylogenetic analysis based on the RdRp sequence strongly suggested that the virus infecting A. brassicicola should be considered a representative of a novel endornavirus species, and this virus was designated as Alternaria brassicicola endornavirus 1 (AbEV1).

Journal

Archives of VirologySpringer Journals

Published: Jul 1, 2015

References

  • A novel mycovirus associated with four double-stranded RNAs affects host fungal growth in Alternaria alternata
    Aoki, N; Moriyama, H; Kodama, M; Arie, T; Teraoka, T; Fukuhara, T
  • The complete genome sequence of a novel mycovirus from Alternaria longipes strain HN28
    Lin, Y; Zhang, H; Zhao, C; Liu, S; Guo, L
  • Detection and sequence analysis of two novel co-infecting double-strand RNA mycoviruses in Ustilaginoidea virens
    Zhong, J; Lei, XH; Zhu, JZ; Song, G; Zhang, YD; Chen, Y; Gao, BD

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