Genome rearrangements in Azospirillum brasilense Sp7 with the involvement of the plasmid pRhico and the prophage ΦAb-Cd

Genome rearrangements in Azospirillum brasilense Sp7 with the involvement of the plasmid pRhico... Alphaproteobacteria of the species Azospirillum brasilense have a multicomponent genome that undergoes frequent spontaneous rearrangements, yielding changes in the plasmid profiles of strains. Specifically, variants (Cd, Sp7.K2, Sp7.1, Sp7.4, Sp7.8, etc.) of the type strain A. brasilense Sp7 that had lost a 115-MDa plasmid were previously selected. In many of them, the molecular weight of a 90-MDa plasmid (p90 or pRhico), which is a kind of “depot” for glycopolymer biosynthesis genes, increased. In this study, a collection of primers was designed to the plasmid pRhico and to the DNA of prophage ΦAb-Cd integrated in it. The use of these primers in polymerase chain reactions allowed the detection of the probable excision of ΦAb-Cd phage from the DNA of A. brasilense variants Sp7.4 and Sp7.8 and other alterations of the pRhico structure in A. brasilense strains Cd, Sp7.K2, and Sp7.8. The developed primers and PCR conditions may be recommended for primary analysis of spontaneous plasmid rearrangements in A. brasilense Sp7 and related strains. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Genome rearrangements in Azospirillum brasilense Sp7 with the involvement of the plasmid pRhico and the prophage ΦAb-Cd

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Publisher
Pleiades Publishing
Copyright
Copyright © 2015 by Pleiades Publishing, Inc.
Subject
Biomedicine; Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795415110095
Publisher site
See Article on Publisher Site

Abstract

Alphaproteobacteria of the species Azospirillum brasilense have a multicomponent genome that undergoes frequent spontaneous rearrangements, yielding changes in the plasmid profiles of strains. Specifically, variants (Cd, Sp7.K2, Sp7.1, Sp7.4, Sp7.8, etc.) of the type strain A. brasilense Sp7 that had lost a 115-MDa plasmid were previously selected. In many of them, the molecular weight of a 90-MDa plasmid (p90 or pRhico), which is a kind of “depot” for glycopolymer biosynthesis genes, increased. In this study, a collection of primers was designed to the plasmid pRhico and to the DNA of prophage ΦAb-Cd integrated in it. The use of these primers in polymerase chain reactions allowed the detection of the probable excision of ΦAb-Cd phage from the DNA of A. brasilense variants Sp7.4 and Sp7.8 and other alterations of the pRhico structure in A. brasilense strains Cd, Sp7.K2, and Sp7.8. The developed primers and PCR conditions may be recommended for primary analysis of spontaneous plasmid rearrangements in A. brasilense Sp7 and related strains.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Dec 16, 2015

References

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