Genome analysis and characterization of a tobacco mosaic virus isolate infecting balsam ( Impatiens balsamina )

Genome analysis and characterization of a tobacco mosaic virus isolate infecting balsam (... The complete RNA genomic sequence of a tobacco mosaic virus isolate infecting Impatiens balsamina , designated as TMV-IM, has been determined. The genomic sequence and the predicted gene products of TMV-IM were similar to those of other members of the genus Tobamovirus. The CP ORF of TMV-IM showed sequence identities of 95.0–99.5% with the corresponding ORFs of other TMV strains. Full-length cDNA of TMV-IM was amplified by RT-PCR with a 5′-end primer harboring a T7 promoter sequence and a 3′-end TMV-specific primer. Subsequently, the full-length cDNA was cloned into plasmid vectors. Capped transcripts synthesized from the cDNA clone were highly infectious and caused characteristic symptoms in balsam plants, similar to wild-type TMV-IM and TMV-U1. These results provide definitive evidence for the natural occurrence of TMV in balsam. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Genome analysis and characterization of a tobacco mosaic virus isolate infecting balsam ( Impatiens balsamina )

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Publisher
Springer Journals
Copyright
Copyright © 2009 by Springer-Verlag
Subject
Biomedicine; Infectious Diseases; Medical Microbiology ; Virology
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-009-0377-x
Publisher site
See Article on Publisher Site

Abstract

The complete RNA genomic sequence of a tobacco mosaic virus isolate infecting Impatiens balsamina , designated as TMV-IM, has been determined. The genomic sequence and the predicted gene products of TMV-IM were similar to those of other members of the genus Tobamovirus. The CP ORF of TMV-IM showed sequence identities of 95.0–99.5% with the corresponding ORFs of other TMV strains. Full-length cDNA of TMV-IM was amplified by RT-PCR with a 5′-end primer harboring a T7 promoter sequence and a 3′-end TMV-specific primer. Subsequently, the full-length cDNA was cloned into plasmid vectors. Capped transcripts synthesized from the cDNA clone were highly infectious and caused characteristic symptoms in balsam plants, similar to wild-type TMV-IM and TMV-U1. These results provide definitive evidence for the natural occurrence of TMV in balsam.

Journal

Archives of VirologySpringer Journals

Published: May 1, 2009

References

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