Genetic variation of eggplant mottled dwarf virus from annual and perennial plant hosts

Genetic variation of eggplant mottled dwarf virus from annual and perennial plant hosts The genetic diversity of eggplant mottled dwarf virus (EMDV), a member of the family Rhabdoviridae , was studied using isolates collected from different herbaceous and woody plant species and remote geographic areas. Sequences corresponding to the N, X, P, Y, M and G ORFs as well as the untranslated regions (UTRs) between ORFs were determined from all isolates. Low genetic diversity was found in almost all genomic regions studied except for the X ORF and the UTRs, which were more variable, while interestingly, an EMDV isolate from caper possessed a truncated G gene sequence. Furthermore, low d N /d S ratios, indicative of purifying selection, were calculated for all genes. Phylogenetic analysis showed that the EMDV isolates clustered in three distinct subgroups based on their geographical origin, with the exception of one subgroup that consisted of isolates from northern Greece and Cyprus. Overall, the level of genetic diversity of EMDV differed between seed- and asexually propagated plants in our collection, and this could be related to the mode of transmission. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Genetic variation of eggplant mottled dwarf virus from annual and perennial plant hosts

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Publisher
Springer Vienna
Copyright
Copyright © 2016 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2705-7
Publisher site
See Article on Publisher Site

Abstract

The genetic diversity of eggplant mottled dwarf virus (EMDV), a member of the family Rhabdoviridae , was studied using isolates collected from different herbaceous and woody plant species and remote geographic areas. Sequences corresponding to the N, X, P, Y, M and G ORFs as well as the untranslated regions (UTRs) between ORFs were determined from all isolates. Low genetic diversity was found in almost all genomic regions studied except for the X ORF and the UTRs, which were more variable, while interestingly, an EMDV isolate from caper possessed a truncated G gene sequence. Furthermore, low d N /d S ratios, indicative of purifying selection, were calculated for all genes. Phylogenetic analysis showed that the EMDV isolates clustered in three distinct subgroups based on their geographical origin, with the exception of one subgroup that consisted of isolates from northern Greece and Cyprus. Overall, the level of genetic diversity of EMDV differed between seed- and asexually propagated plants in our collection, and this could be related to the mode of transmission.

Journal

Archives of VirologySpringer Journals

Published: Mar 1, 2016

References

  • Complete genome sequence and genome analysis of Eggplant mottled dwarf virus-Iranian Isolate
    Babaie, G; Kouhi, Habibi M; Massah, A; Dizadji, A; Izadinejad, L; Simon, A
  • Nucleocapsid gene variability reveals two subgroups of Lettuce necrotic yellows virus
    Callaghan, B; Dietzgen, RG

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