Genetic variation in a sorghum line with multiple genetic instability induced with ethidium bromide in an in vitro culture

Genetic variation in a sorghum line with multiple genetic instability induced with ethidium... Ethidium bromide treatment (15 mg/l, 26°C, 18 h) of a sorghum Zheltozernoe 10 callus culture yielded line Zh10-br1 displaying multiple genetic instability. The line was characterized by a broad variety of mutations, which were identified in consecutive generations obtained from one initial regenerant via self-pollination. The mutations caused male sterility (male sterility, generation R1), a low plant height (dwarfness, R2), a reduced awn length (awnless, R3), yellow leaves in seedlings (xantha, R6), leaf variegation (leaf variegation, R6), leaf bleaching (virescence, R6), etc. In some cases, segregation in families suggested a monogenic recessive inheritance for the induced mutations. Male sterility was due to a range of defects that affected microsporogenesis and microgametogenesis and were probably caused by mutations of several genes. Leaf variegation was due to the appearance of green sectors in originally albino seedling leaves; the reversion occurred only in somatic tissues without affecting male and female gametes. In male-sterile and variegated mutants, sequence-specific amplified polymorphism analysis with primers to the Isaak transposon revealed new DNA fragments, which were absent from the original line. The results supported the hypothesis that the mutations isolated in line Zh10-br1 result from transposon mobilization induced by ethidium bromide and/or in vitro culture conditions. Russian Journal of Genetics Springer Journals

Genetic variation in a sorghum line with multiple genetic instability induced with ethidium bromide in an in vitro culture

Loading next page...
SP MAIK Nauka/Interperiodica
Copyright © 2010 by Pleiades Publishing, Ltd.
Biomedicine; Microbial Genetics and Genomics; Animal Genetics and Genomics; Human Genetics
Publisher site
See Article on Publisher Site


You’re reading a free preview. Subscribe to read the entire article.

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches


Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.



billed annually
Start Free Trial

14-day Free Trial