Genetic stability of recombinant potato virus X virus vectors presenting foreign epitopes

Genetic stability of recombinant potato virus X virus vectors presenting foreign epitopes We investigated the genetic stability of recombinant potato virus X vectors presenting beet necrotic yellow vein virus (BNYVV) epitopes. Following N-terminal PVX coat protein (CP) fusion of the BNYVV epitopes, we inoculated Nicotiana benthamiana plants with recombinant (r)PVX and carried out five serial passages through systemically-infected plants. RT-PCR investigation of the BNYVV epitope sequences revealed the accumulation of several point mutations and deletions, predominantly affecting positively-charged residues. A comparison of the isoelectric point (pI) values and charges of the wild type and rCPs showed that the initial high rCP pI values had changed to values closer to that of the wild-type CP. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Genetic stability of recombinant potato virus X virus vectors presenting foreign epitopes

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Publisher
Springer-Verlag
Copyright
Copyright © 2007 by Springer-Verlag
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-006-0892-y
Publisher site
See Article on Publisher Site

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