Genetic relationships among resynthesized, semi-
resynthesized and natural Brassica napus L. genotypes
Wojciech M. Karłowski
Received: 26 May 2017 / Accepted: 10 August 2017
Ó Springer Science+Business Media B.V. 2017
Abstract The allopolyploidization event that created
cultivated oilseed rape Brassica napus L, followed by
intense breeding, reduced its genetic diversity. Resyn-
thesized (RS) B. napus L. obtained by interspeciﬁc
hybridization between genotypes of B. rapa L. and B.
oleracea L. can be a valuable source for broadening
genetic diversity in cultivated oilseed rape. In this study,
we determined the extent of DNA polymorphism among
natural accessions of oilseed rape, resynthesized B.
napus, their parental species and double-low quality
semi-RS lines carrying the Rfo gene. Using 10 selected
primer combinations, 522 polymorphic AFLP markers
were scored in the complete set of 100 Brassica sp. To
detect relationships between these genotypes, a cluster
analysis was performed using the Jaccard’s distance.
Resynthesized allopolyploids clustered directly
between their diploid parents. Cultivated accessions of
oilseed rape created a compact group away from
resynthesized allopolyploids as well as semi-RS lines.
The natural oilseed rape group, which consists of 49
cultivars and breeding lines of oilseed rape, is charac-
terized by lower genetic diversity than the group of 33
accessions of resynthesized oilseed rape, and the
analysis showed that the double-low quality semi-RS
lines represent a speciﬁc genetic variation of B. napus.
The de novo resynthesized B. napus lines and the semi-
RS lines of double-low quality generated from them,
provide a signiﬁcant opportunity for enrichment the
gene pool of oilseed rape.
Keywords Brassica napus Á Resynthesis Á Semi-
resynthesis Á Genetic distance Á Genetic diversity
RS Resynthesized Brassica napus
Semi-resynthesized line (line obtained by
crossing oilseed rape 9 RS)
DH Doubled haploid
Brassica napus seed quality types
‘‘ ??’’ High erucic acid, high glucosinolate
‘‘ 0 ?’’ Low erucic acid, high glucosinolate
Electronic supplementary material The online version of
this article (doi:10.1007/s10681-017-2000-7) contains supple-
mentary material, which is available to authorized users.
K. Sosnowska (&) Á T. Cegielska-Taras Á
A. Liersch Á L. Szała Á K. Mikołajczyk Á W. Popławska
Department of Genetics and Breeding of Oilseed Crops,
Plant Breeding and Acclimatization Institute-National
Research Institute, Strzeszyn
ska 36, 60-479 Poznan
W. M. Karłowski
Department of Computational Biology, Institute of
Molecular Biology and Biotechnology, Adam Mickiewicz
Department of Mathematical and Statistical Methods,
University of Life Sciences, Poznan
Euphytica (2017) 213:212