540 Mammalian Genome 8, Brief Data Reports Segregation of RFLVs in an interspecific mouse backcross Database deposit information: Genebank Accession Number: panel allowed the assignment of two loci. One locus occurs on Chr U89503 11 in a region with previously established homology to human Chr Molecular reagents used for mapping: A 1.3-kb hybridization 17, consistent with the location of human profilin 1 (PFN1; see probe containing exon 10 of the human BRCA2 gene [2,3] was generated by PCR (5'-TTTCTATGAGAAAGGTTGTGAG and Fig. 1). Our mapping data thus place the Pfnl gene near previously mapped mutations including oe (open eyelid), co (cocked), and 5'-AGCAGAAAAAAACACAGAAGGA). This probe was used Mdsh (Miller-Dieker syndrome homolog). Interestingly, in some to identify a Bacterial Artificial Chromosome clone, (593D1), by humans with Miller-Dieker syndrome, the PFN1 gene is deleted, hybridization screening of high density filters containing the CJ7 but in others it appears intact . Thus, allelic deletion of the embryonic stem cell line library (Research Genetics, Huntsville, AL) derived from a 129Sv mouse. Subsequently, a 407-bp EcoRV profilin locus may contribute to the clinical phenotype of Miller- Dieker syndrome in some patients, but does not determine the fragment from the BAC 593D1 bacterial artificial chromosome essential
Mammalian Genome – Springer Journals
Published: Mar 24, 2009
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