Mammalian Genome 12, 180–182 (2001). DOI: 10.1007/s003350010245 Incorporating Mouse Genome © Springer-Verlag New York Inc. 2001 Genetic mapping of spinal dysmyelination in cross-bred American Brown Swiss cattle to bovine Chromosome 11 1,2 1 3 2 1 Peter H. Nissen, Naseer M. Shukri, Jørgen S. Agerholm, Merete Fredholm, Christian Bendixen Danish Institute of Agricultural Sciences, Department of Animal Breeding and Genetics, DK-8830 Tjele, Denmark Royal Veterinary and Agricultural University, Department of Animal Genetics and Breeding, Grønnegårdsvej 3, DK-1870 Frederiksberg, Denmark Danish Veterinary Laboratory, Department of Pathology and Epidemiology, Bu ¨ lowsvej 27, DK-1790 Copenhagen, Denmark Received: 2 June 2000 / Accepted: 18 September 2000 Bovine spinal dysmyelination (BSD) is a congenital neurological formed in duplexes in a volume of 5 ml in an ABI 877 PCR robot disorder occurring in several national cattle breeds upgraded with (Applied Biosystems), containing 12 ng genomic DNA,1×PCR American Brown Swiss (ABS) cattle. The disease was first de- buffer, 0.4 U AmpliTaq Gold (Applied Biosystems), 20 pmol of scribed in 1993 (Hafner et al. 1993), and in the Red Danish Dairy each primer, and 2.0 mM MgCl . All markers were run under the breed the number of affected calves peaked in 1994 with 163
Mammalian Genome – Springer Journals
Published: Feb 1, 2001
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