Genetic mapping of 10 microsatellites in the t complex region of mouse Chromosome 17

Genetic mapping of 10 microsatellites in the t complex region of mouse Chromosome 17 Mammalian Genome 9, 472 (1998). Incorporating Mouse Genome © Springer-Verlag New York Inc. 1998 Genetic mapping of 10 microsatellites in the t complex region of mouse Chromosome 17 1 2 1 Corine Vernet, Kuniya Abe, Karen Artzt Cell and Molecular Biology Institute, the University of Texas at Austin, Austin, Texas 78712-1064, USA Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Kumamoto 862, Japan Received: 17 December 1997 / Accepted: 24 February 1998 A complete t haplotype consists of four large non-overlapping inversions of mouse Chromosome (Chr) 17 with respect to the wild-type homolog and is naturally occurring in wild mouse popu- lations by recombination suppression and transmission ratio dis- tortion (Silver et al. 1992). This t complex includes the major histocompatibility complex (H2), the loci of Brachyury (T) and tufted (tf), as well as several lethal mutations like tclw5 and tcl12 (Hamvas et al. 1997). The tclw5 mutation is an early acting em- bryonic lethal, recombinationally inseparable from H2-K (Shin et al. 1984). In an attempt to positionally clone tclw5, contigs of cosmids have been made, 18 genes have been identified, and a detailed description of the region is now available (Abe et al. 1988; Yeom et al. 1992; Lai et al. 1994). Unfortunately, the restricted polymor- phism of probes and the size of the region to explore have made the approach particularly tedious. When the first microsatellites (Mits) became available (Dietrich et al. 1994), they proved to be extremely useful for our linkage analysis project: we refined the x y mapping of 12 Mits, using our panel of 140 t /t recombinants and narrowed down the genetic region containing tclw5 (Vernet and Artzt 1995). In the present report, using the same panel, we have refined the location of 10 new satellites from the second release of microsatellites and define now a new genetic limit to tclw5. Mit primer pairs for the polymerase chain reaction (PCR) were purchased from Research Genetics (Huntsville, Ala.); isolated DNA from recombinant mice was amplified under conditions de- scribed by Dietrich and co-workers (1994). Amplified bands were resolved on 6% denaturating polyacrylamide electrophoresis gels. Figure 1 shows a summary of the Mit map and the number of Fig. 1. Map of mouse t haplotype Chr 17 between H2D and tf. Number of mice recombinant for a locus and the locus immediately above is shown on recombinants between each locus. The t recombinant crosses were the left side of the map. The new markers mapped and the telomeric limit able to separate several markers that previously were considered to D17Mit147 are written in bold. be in the same bin. Additionally, D17Mit147 is the new telomeric limit for the region where tclw5 is located. Hamvas RMJ, Artzt K, Fischer-Lindahl K, Trachtulec Z, Vernet C et al. Acknowledgments. This work was supported by NICHD grants HD30658 (1997) Mouse Chromosome 17. Mamm Genome 7 (Suppl), S274–S294 and HD10668 to K.A. Lai F, Stubbs L, Lehrach H, Huang Y, Yeom YI et al. (1994) Genomic organization and expressed sequences of the mouse extended H-2K re- gion. Genomics 23, 338–343 References Shin H-S, Bennett D, Artzt K (1984) Gene mapping within the T/t-complex of the mouse. IV. The inverted MHC is intermingled with several t-lethal Abe K, Wei J-F, Wei F-S, Hsu Y-C, Uehara H et al. (1988) Searching for genes. Cell 39, 573–578 coding sequences in the mammalian genome: the H-2K region of the Silver LM, Artzt K, Barlow D, Fischer Lindahl K, Lyon MF et al. (1992) mouse MHC is replete with genes expressed in embryos. EMBO J 7, Mouse Chromosome 17. Mamm Genome 3 (Suppl), S241–S260 3441–3449 Vernet C, Artzt K (1995) Mapping of 12 markers in the proximal region of Dietrich WF, Miller JC, Steen RG, Merchant M, Damron D et al. (1994) mouse Chromosome 17 using recombinant t haplotypes. Mamm Ge- A genetic map of the mouse with 4,006 simple sequence length poly- nome 6, 219–221 morphisms. Nat Genet 7, 220–245 Yeom YI, Abe K, Bennett D, Artzt K (1992) Testis-/embryo-expressed genes are clustered in the mouse H-2K region. Proc Natl Acad Sci USA Correspondence to: C. Vernet 89, 773–777 Mammalian Genome Springer Journals

Genetic mapping of 10 microsatellites in the t complex region of mouse Chromosome 17

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Copyright © 1998 by Springer-Verlag New York Inc.
Life Sciences; Cell Biology; Animal Genetics and Genomics; Human Genetics
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