ISSN 1022-7954, Russian Journal of Genetics, 2006, Vol. 42, No. 3, pp. 311–316. © Pleiades Publishing, Inc., 2006.
cies and subspecies is fundamental to the improvement
of breeding wheat. Some species among Triticum L.
had been investigated, including T. durum , T. aethi-
opicum , T. spelta , T. macha , T. dicoccoides
[6, 7], T. polonicum , and
T. dicoccon .
Turgidum wheat, Triticum turgidum L. (2n = 4x = 28;
AABB), had been widely cultivated in some countries
and areas, including Spain, France, Italy, and China. In
China, turgidum wheat was grown in wide areas of the
country before 1950s; however, its cultivation area has
gradually reduced and only cultivated in southwest
China since 1980
s . The turgidum wheats from
China have been characterized on C-banding , Wax
subunits , gliadin and HMW-glutenin , esterase
, quality  and the crossability with rye .
However, the molecular genetic diversity of turgidum
wheat, especially the turgidum wheat landraces from
southwest of China, was still unknown. Microsatellites,
as simple sequence repeats (SSR), are shown
ubiquitous, and found high polymorphism both in cod-
ing and noncoding regions in genomes [17–19]. SSR
have been extensively exploited as DNA markers for the
evaluation of wheat cultivars or landraces [20, 21]. The
object of this study was to estimate the genetic diversity
in turgidum wheat from southwest China, at the DNA
MATERIALS AND METHODS
Plant material. A total of 48 T. turgidum L. acces-
sions, including 30 T. turgidum L. ssp. turgidum acces-
sions, 7 T. turgidum L. ssp. durum accessions, 4 T. tur-
gidum L. ssp. carthlicum accessions, 3 T. turgidum L.
ssp. paleocolchicum accessions, 2 T. turgidum L. ssp.
turanicum accessions and 2
T. turgidum L. ssp. poloni-
cum accessions, were used in this study (Table 1),
among which 37 T. turgidum L. accessions are derived
Genetic Diversity of Triticum turgidum L.
Based on Microsatellite Markers*
1, 2, 3
, D.-F. Zhang
, Y.-M. Wei
, Z.-H. Yan
, and Y.-L. Zheng
Ministry of Education Key Laboratory of Southwest Crop Genetic Resources and Improvement, Sichuan Agricultural University,
Yaan, Sichuan, 625014 China; fax: (86-835)288-31-53; e-mail: firstname.lastname@example.org
Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, Sichuan, 611830 China
College of Agronomy, Sichuan Agricultural University, Yaan, Sichuan, 625014 China
Received September 19, 2005
Abstract—Using microsatellite (SSR) markers, the genetic diversity and genetic relationships among 48 Triti-
cum turgidum L. accessions, including 30 Triticum turgidum L. ssp. turgidum, 7 Triticum turgidum L. ssp.
durum, 4 Triticum turgidum L. ssp. carthlicum, 3 Triticum turgidum L. ssp. paleocolchicum, 2 Triticum turgi-
dum L. ssp. turanicum, and 2 Triticum turgidum L. ssp. polonicum accessions, were investigated. A total of 97
alleles were detected at 16 SSR loci. At each locus, the number of alleles ranged from two to fourteen, with an
average of 6.1. The genetic similarity (GS) value ranged from 0.20 to 0.92, with the mean of 0.59. In cluster
analysis, it was found the 48 Triticum turgidum L. accessions could be distinguished easily by SSR markers,
whereas the six subspecies taxonomic entities of T. turgidum L. could not differentiate with each other, indicat-
ing that the morphological differences present among the six subspecies could not be reﬂected by the SSR
markers. These results suggested that SSR markers had superiority in detecting the genetic diversity of T. tur-
gidum L., while they were not good for studies of the phylogenic relationships among the subspecies of T. tur-
* The text was submitted by the authors in English.