Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You and Your Team.

Learn More →

Genetic Diversity of Persian Ecotypes of Indian Walnut (Aeluropus littoralis (Gouan) Pari.) by AFLP and ISSR Markers

Genetic Diversity of Persian Ecotypes of Indian Walnut (Aeluropus littoralis (Gouan) Pari.) by... Indian walnut Aeluropus littoralis (Gouan) Pari, well known for its salt tolerance and forage quality, is a perennial monocot grass from Chlorideae tribe that shows a high potential to become an important genetic model. Although, Aeluropus germplasm is very rich in Iran; but is still unexplored. In this study, 20 ecotypes of A. littoralis were gathered from different geographic zones and a set 16 primers (ISSRs-AFLPs combined) was used for genetic diversity evaluation. A total of 635 fragments and 594 polymorphic fragments were produced (93.5%) and modest levels of genetic similarity were found (ranging from 0.38 to 0.71) among ecotypes. The results showed an equal competition between the two markers on polymorphism detection, so both molecular techniques were able to distinguish the A. littoralis ecotypes. Furthermore, Dendrogram based on both the Bayesian and PCoA analysis showed a clear discrimination and significant variation among ecotypes; however, molecular clustering indicated a weak concordance with geographical grouping. The amount of molecular diversity revealed by molecular markers for A. littoralis in this study can be exploited for pasture conservation programs and new forage crop development. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Cytology and Genetics Springer Journals

Genetic Diversity of Persian Ecotypes of Indian Walnut (Aeluropus littoralis (Gouan) Pari.) by AFLP and ISSR Markers

Download PDF
9 pages
Read Article

Loading next page...
 
/lp/springer_journal/genetic-diversity-of-persian-ecotypes-of-indian-walnut-aeluropus-CgFn5bpzu6
Publisher
Springer Journals
Copyright
Copyright © 2018 by Allerton Press, Inc.
Subject
Biomedicine; Human Genetics
ISSN
0095-4527
eISSN
1934-9440
DOI
10.3103/S009545271803012X
Publisher site
See Article on Publisher Site

Abstract

Indian walnut Aeluropus littoralis (Gouan) Pari, well known for its salt tolerance and forage quality, is a perennial monocot grass from Chlorideae tribe that shows a high potential to become an important genetic model. Although, Aeluropus germplasm is very rich in Iran; but is still unexplored. In this study, 20 ecotypes of A. littoralis were gathered from different geographic zones and a set 16 primers (ISSRs-AFLPs combined) was used for genetic diversity evaluation. A total of 635 fragments and 594 polymorphic fragments were produced (93.5%) and modest levels of genetic similarity were found (ranging from 0.38 to 0.71) among ecotypes. The results showed an equal competition between the two markers on polymorphism detection, so both molecular techniques were able to distinguish the A. littoralis ecotypes. Furthermore, Dendrogram based on both the Bayesian and PCoA analysis showed a clear discrimination and significant variation among ecotypes; however, molecular clustering indicated a weak concordance with geographical grouping. The amount of molecular diversity revealed by molecular markers for A. littoralis in this study can be exploited for pasture conservation programs and new forage crop development.

Journal

Cytology and Genetics – Springer Journals

Published: Jun 4, 2018

References

  • Ultrastructure of Aeluropus littoralis leaf salt glands under NaCl stress
    Barhoumi, Z.; Djebali, W.; Abdelly, C.; Chabi, W.; Smaoui, A.
  • Salt impact on photosynthesis and leaf ultrastructure of Aeluropus littoralis
    Barhoumi, Z.; Djebali, W.; Chabi, W.; Abdelly, C.; Smaoui, A.
  • Contribution of NaCl excretion to salt resistance of Aeluropus littoralis (Willd) Parl
    Barhoumi, Z.; Djebali, W.; Smaoui, A.; Chabi, W.; Abdelly, C.
  • Saltinduced reduction in shoot spermine pool of Aeluropus littoralis
    Nasiri, N.; Shokri, E.; Nematzadeh, G.H.
  • Aeluropus littoralis NaCl induced vacuolar H+-ATPase subunit c: molecular cloning and expression analysis
    Nasiri, N.; Shokri, E.; Nematzadeh, G.H.
  • Characterization and expression of a vacuolar Na+/H+ antiporter gene from the monocot halophyte Aeluropus littoralis
    Zhang, G.H.; Su, Q.; An, L.J.; Wu, S.
  • Codon bias patterns in photosynthetic genes of halophytic grass Aeluropus littoralis
    Younesi-Melerdi, E.; Nematzadeh, G.H.; Shokri, E.
  • Biochemical responses of Gouan (Aeluropus littoralis) to heavy metals stress
    Rastgoo, L.; Alemzade, A.
  • Expression of the Aeluropus littoralis AlSAP gene in rice confers broad tolerance to abiotic stresses through maintenance of photosynthesis, Plant
    Ben Saad, R.; Fabre, D.; Mieulet, D.; Meynard, D.; Dingkuhn, M.; Al-Doss, A.; Guiderdoni, E.; Hassairi, A.
  • Cloning and functional expression in Saccharomyces cereviae of a K+ transporter
    Su, Q.; Feng, S.; An, L.; Zhang, G.
  • Transfer of salt tolerance from Aeluropus littoralis sinensis to wheat (Triticum aestium L.) via asymmetric somatic hybridization
    Wei, Y.; Guangmin, X.; Daying, Z.; Huimin, C.
  • Identification and sequencing of ESTs from the halophyte grass Aeluropus littoralis
    Zouari, N.; Ben Saad, R.; Legavre, T.; Azaza, J.; Sabau, X.; Jaoua, M.; Asmoudi, K.; Hassairi, A.
  • Forage quality of two halophytic species
    Sharifi-Rad, M.; Sharifi-Rad, J.; Jaime, A.; Mohsenzadeh, S.
  • Determination of nutritive value of five species of halophyte plants used by camel in East South Iran
    Yousef-Elahi, M.
  • AFLP: a new technique for DNA fingerprinting
    Vos, P.; Hogers, R.; Bleeker, M.; Reijans, M.; Lee, T.; Hornes, M.; Frijters, A.; Pot, J.; Peleman, J.; Kuiper, M.; Zabeau, M.
  • Dna polymorphisms amplified by arbitrary primers are useful as genetic markers
    Williams, J.G.; Kubelik, A.R.; Livak, K.J.; Rafalski, A.J.; Tingey, S.V.
  • Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification
    Zietkiewicz, E.; Rafalski, A.; Labuda, D.
  • Cross species amplification of Pennisetum glaucum microsatellite markers in Pennisetum purpureum and genetic diversity of Napier grass accessions
    Azevedo, A.L.S.; Costa, P.P.; Machado, J.C.; Machado, M.A.; Pereira, A.V.; Silva Ledo, F.J.
  • Genome diversity in wild grasses under environmental stress
    Fitzgerald, T.L.; Shapter, F.M.; McDonald, S.; Waters, D.L.; Chivers, I.H.; Drenth, I.A.; Nevo, E.; Henry, R.J.
  • Genetic diversity of fringed brome (Bromus ciliatus) as determined by amplified fragment length polymorphism
    Fu, Y.B.; Coulman, B.E.; Ferdinandez, Y.S.N.; Cayouette, J.; Peterson, P.M.
  • Assessment of genetic diversity in Napier grass (Pennisetum purpureum Schum.) using microsatellite, single-nucleotide polymorphism and insertion-deletion markers from pearl millet (Pennisetum glaucum [l.] R. Br.)
    Kandel, R.; Hari, P.; Bharat, P.; Karen, R.; Harris-Shultz, S.; Anderson, W.F.
  • Genetic variation in remnant Festuca hallii populations is weakly differentiated, but geographically associated across the Canadian Prairie
    Qiu, J.; Fu, Y.B.; Bai, Y.; Wilmshurst, J.F.
  • Evaluation of the DNA fingerprinting method AFLP as a new tool in bacterial taxonomy
    Janssen, P.; Coopman, R.; Huys, G.; Swings, J.; Bleeker, M.; Vos, P.; Zabeau, M.; Kersters, K.
  • Genetic relationships among South American species of Cunila D. Royen ex L. based on ISSR
    Agostini, G.; Echeverrigaray, S.; Souza-Chies, T.T.
  • Comparative analysis of seeded and vegetative biotype buffalo grasses based on phylogenetic relationship using ISSRs
    Budak, H.; Shearman, R.C.; Parmaksiz, I.; Dweikat, I.
  • Correspondence of ISSR and RAPD markers for comparative analysis of genetic diversity among different apricot genotypes from cold arid deserts of trans-Himalayas
    Kumar, M.; Mishra, G.P.; Singh, R.; Kumar, J.; Naik, P.K.; Singh, S.B.
  • Assessing genetic diversity of Elymus sibiricus (Poaceae: Triticeae) populations from Qinghai-Tibet Plateau by ISSR markers
    Ma, X.; Zhang, X.Q.; Zhou, Y.H.; Bai, S.Q.; Liu, W.
  • The extent of genetic diversity among Vanilla species: comparative results for RAPD and ISSR
    Verma, P.C.; Chakrabarty, D.; Jena, S.N.; Mishra, D.K.; Singh, P.K.; Sawant, S.V.; Tuli, R.
  • Genetic diversity and relationship of endangered plant Magnolia officinalis (Magnoliaceae) assessed with ISSR polymorphisms
    Yu, H.H.; Yang, Z.L.; Sun, B.; Liu, R.N.
  • Use of ISSR fingerprints to detect microsatellites and genetic diversity in several related Brassica taxa and Arabidopsis thaliana
    Bornet, B.; Branchard, M.
  • Comparative assessment of DNA fingerprinting techniques (RAPD
    Archak, S.; Gaikwad, A.B.; Gautam, D.; Rao, E.V.; Swamy, K.R.; Karihaloo, J.L.
  • Molecular marker-based characterization in candidate plus trees of Pongamia pinnata, a potential biodiesel legume
    Kesari, V.; Sathyanarayana, V.M.; Parida, A.; Rangan, L.
  • Assessment of genetic diversity through RAPD
    Naik, P.K.; Alam, M.F.; Singh, H.; Goyal, V.; Parida, S.; Kalia, S.; Mohapatra, T.
  • Assaying polymorphism at DNA level for genetic diversity diagnostics of the safflower (Carthamus tinctorius L.) world germplasm resources
    Sehgal, D.; Rajpal, V.R.; Raina, S.N.; Sasanuma, T.; Sasakuma, T.
  • A plant molecular DNA mini preparation: version II
    Dellaporta, S.L.; Wood, J.; Tickes, J.B.
  • Evaluation of TYLCVresistant tomato germplasm using molecular markers
    Park, Y.H.; Kim, K.H.; Choi, Y.M.; Choi, H.S.; Chae, Y.; Park, K.S.; Chung, S.M.
  • Optimization of ISSR markers for African edible-seeded Cucurbitaceae species’ genetic diversity analysis
    Dje, Y.; Tahi, G.C.; ZoroBi, I.A.; Malice, M.; Baudoin, J.P.; Bertin, P.
  • The comparison of RFLP
    Powell, W.; Morgante, M.; Andre, C.; Hanafey, M.; Vogel, J.; Tingey, S.; Rafalski, A.
  • AFLP-based molecular characterization and population structure analysis of Silybum marianum L
    Mohammadi, S.A.; Shokrpour, M.; Moghaddam, M.; Javanshir, A.
  • Measures of the amount of ecologic association between species
    Dice, L.R.
  • Mathematical model for studying genetic variation in terms of restriction endonucleases
    Nei, M.; Li, W.H.
  • NTSYS-pc: Version 2.02. Numerical taxonomy and multivariate analysis system
    Rohlf, F.J.
  • Detection of disease, clustering and generalized regression approach
    Mantel, N.
  • Documentation for STRUCTURE software
    Pritchard, J.K.; Wen, W.; Falush, D.
  • GenAlEx 6.5: genetic analysis in Excel
    Peakall, R.; Smouse, P.E.
  • Comparative analysis of phylogenetic relationships of grain amaranths and their wild relatives (Amaranthus; Amaranthaceae) using internal transcribed spacer, amplified fragment length polymorphism, and double-primer fluorescent intersimple sequence repeat markers
    Xu, F.; Sun, M.
  • Genetic relationship among selected crested wheatgrass cultivars and species determined on the basis of AFLP markers
    Mellish, A.; Coulman, B.; Ferdinandez, Y.
  • Evaluating genetic variation and relationships among two bromegrass species and their hybrid using RAPD and Aflp markers
    Ferdinandez, Y.; Coulman, B.E.
  • Patterns of amplified restriction fragment polymorphism in natural populations and corresponding seed collections of plains rough fescue
    Qiu, J.; Fu, Y.B.; Bai, Y.; Wilmshurst, J.F.
  • Genetic diversity patterns in Phragmites australis at the population, regional and continental scales
    Lambertini, C.; Gustafsson, M.; Frydenberg, J.; Speranza, M.; Brix, H.
  • Population diversity of Aleuropus lagopoides: a potential cash crop for saline land
    Ahmed, M.Z.; Gilani, S.A.; Kikuchi, A.; Gulzar, S.; Khan, M.A.; Watanabe, K.N.
  • Aeluropus petereganicus (Poaceae), a new species from Iran
    Khodashenas, M.
  • Genetic relationships among smooth bromegrass cultivars of different ecotypes detected by AFLP markers
    Ferdinandez, Y.; Coulman, B.E.
  • Seed germination of a halophytic grass Aeluropus lagopoides
    Gulzar, S.; Khan, M.A.
  • Light, salinity and temperature effects on the seed germination of perennial grasses
    Khan, M.A.; Gulzar, S.