Genetic Diversity of Apple Cultivars Growing in Kazakhstan

Genetic Diversity of Apple Cultivars Growing in Kazakhstan Identification of the varieties is the primary requirement for characterization of the gene pool in agricultural production and implementation of breeding programs. In the present work, a set of six SSR markers was used for identification of cultural material collected on various horticultural farms in Kazakhstan: 30 varieties of Kazakhstani selection, 40 foreign varieties, and 16 Dzhangaliev’s apple clones selected in wild apple populations. Values of expected (H e) and observed (H o) heterozygosity in groups of all analyzed varieties were high: from 0.735 to 0.812 and from 0.661 to 0.721 respectively. Cluster analysis and analysis of genetic distances (STRUCTURE, UPGMA) showed a distribution of all samples into four major groups with a set of small subgroups caused by origin diversity. The first group included Kazakhstani varieties originating from Reneitte Burchardt, the second group included varieties with Aporta in parentage, the third group was represented by subclusters with a majority of foreign varieties, and the last group was composed of Dzhangaliev’s apple clones and Kazakhstani varieties with wild apple as their ancestor. Genotyping revealed inconsistencies in individual samples (and/or parentage) with claimed names. Analysis by markers of the Md-ACS1 and Md-ACO1 genes responsible for ethylene levels in fruits, which according to the literature correlates with fruit hardness and storability, did not reveal among Kazakhstani varieties any ACS1-2/2 homozygotes, the genotype with the highest expression of these traits. A quarter of Kazakhstani varieties and about a half of foreign varieties were heterozygous in ACS1, an indication of medium hardness and relatively long period of fruit storage. In two Kazakhstani and two foreign varieties heterozygous in ACS1, an improvement of the traits is possible owing to homozygosity in ACO1-1/1. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Genetic Diversity of Apple Cultivars Growing in Kazakhstan

Loading next page...
 
/lp/springer_journal/genetic-diversity-of-apple-cultivars-growing-in-kazakhstan-xvoJB6OG6s
Publisher
Pleiades Publishing
Copyright
Copyright © 2018 by Pleiades Publishing, Inc.
Subject
Biomedicine; Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1134/S1022795418020138
Publisher site
See Article on Publisher Site

Abstract

Identification of the varieties is the primary requirement for characterization of the gene pool in agricultural production and implementation of breeding programs. In the present work, a set of six SSR markers was used for identification of cultural material collected on various horticultural farms in Kazakhstan: 30 varieties of Kazakhstani selection, 40 foreign varieties, and 16 Dzhangaliev’s apple clones selected in wild apple populations. Values of expected (H e) and observed (H o) heterozygosity in groups of all analyzed varieties were high: from 0.735 to 0.812 and from 0.661 to 0.721 respectively. Cluster analysis and analysis of genetic distances (STRUCTURE, UPGMA) showed a distribution of all samples into four major groups with a set of small subgroups caused by origin diversity. The first group included Kazakhstani varieties originating from Reneitte Burchardt, the second group included varieties with Aporta in parentage, the third group was represented by subclusters with a majority of foreign varieties, and the last group was composed of Dzhangaliev’s apple clones and Kazakhstani varieties with wild apple as their ancestor. Genotyping revealed inconsistencies in individual samples (and/or parentage) with claimed names. Analysis by markers of the Md-ACS1 and Md-ACO1 genes responsible for ethylene levels in fruits, which according to the literature correlates with fruit hardness and storability, did not reveal among Kazakhstani varieties any ACS1-2/2 homozygotes, the genotype with the highest expression of these traits. A quarter of Kazakhstani varieties and about a half of foreign varieties were heterozygous in ACS1, an indication of medium hardness and relatively long period of fruit storage. In two Kazakhstani and two foreign varieties heterozygous in ACS1, an improvement of the traits is possible owing to homozygosity in ACO1-1/1.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Mar 12, 2018

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial