Twenty-eight accessions of Rajmash (Phaseolus vulgaris L.), an economically important legume, were collected from Rajouri–Poonch region of J&K State of India to study the extent and distribution of genetic diversity using inter simple sequence repeat markers. Qualitative morphological trait analysis indicated significant amount of variability at seed and seedling stage. Results showed that Nei’s genetic diversity (H) and Shannon’s information indices (I) were highest for Buddhal group of accessions (H = 0.2228; I = 0.3200), and lowest for Loran Mandi group of accessions (H = 0.0272; I = 0.0377). Shannon index based analysis calculated the total species diversity (H sp ) 0.1240 and average diversity within groups of accessions (intra-site) (H pop ) 0.2180. The proportion of diversity among groups of accessions (inter-site) (G ST ) was 0.4311. Within group diversity was 0.5689, i.e. 56.89 % of the total diversity. Analysis of molecular variance showed similar results with 75 % variation existing within groups. Overall, Loran Mandi group of accessions had the greatest distance as revealed by F ST distances and Nei’s unbiased measure of genetic distance. The germplasm studies reveal moderate to high level of genetic diversity and the potential use of P. vulgaris accessions for future crop improvement programmes.
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences – Springer Journals
Published: May 27, 2016
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
All the latest content is available, no embargo periods.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud