Twenty-eight accessions of Rajmash (Phaseolus vulgaris L.), an economically important legume, were collected from Rajouri–Poonch region of J&K State of India to study the extent and distribution of genetic diversity using inter simple sequence repeat markers. Qualitative morphological trait analysis indicated significant amount of variability at seed and seedling stage. Results showed that Nei’s genetic diversity (H) and Shannon’s information indices (I) were highest for Buddhal group of accessions (H = 0.2228; I = 0.3200), and lowest for Loran Mandi group of accessions (H = 0.0272; I = 0.0377). Shannon index based analysis calculated the total species diversity (H sp ) 0.1240 and average diversity within groups of accessions (intra-site) (H pop ) 0.2180. The proportion of diversity among groups of accessions (inter-site) (G ST ) was 0.4311. Within group diversity was 0.5689, i.e. 56.89 % of the total diversity. Analysis of molecular variance showed similar results with 75 % variation existing within groups. Overall, Loran Mandi group of accessions had the greatest distance as revealed by F ST distances and Nei’s unbiased measure of genetic distance. The germplasm studies reveal moderate to high level of genetic diversity and the potential use of P. vulgaris accessions for future crop improvement programmes.
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences – Springer Journals
Published: May 27, 2016
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