1022-7954/04/4012- © 2004
Russian Journal of Genetics, Vol. 40, No. 12, 2004, pp. 1407–1412. Translated from Genetika, Vol. 40, No. 12, 2004, pp. 1695–1701.
Original Russian Text Copyright © 2004 by Pavlov, Kolesnikov, Melnikova, Ushakova.
Analysis of population structure in complex poly-
morphic species is fundamental for understanding the
mechanisms of speciation and microevolution. The
studies of mykizha (
from Kamchatka were for a long time mostly devoted
to phenetics and ecological diversity, which is rather
high in the American  and Asiatic [2–4] parts of the
area. However, the accumulated evidence on numerous
diverse forms were sometimes poorly correlated with the
population genetic structure of this species. Even in early
studies [5–8], analyses of karyology and allozyme varia-
tion in different species revealed much lower diversity.
Now it is clear that most of these differences depend on
speciﬁc features of the species distribution [9–11]. The
genetic diversity of
native to Kamchatka seems to
be lower than that of the North American populations
of this species. This suggestion
needs to be veriﬁed using various genetic markers.
Development of the techniques that operate directly
with nuclear and mitochondrial DNA gave a stimulus
for numerous population genetic studies. Most such
were performed on
the American populations [10, 13–16].
This study is the ﬁrst attempt at evaluating the
intraspeciﬁc variation of the mtDNA genome in
native to Kamchatka and in poorly studied
MATERIALS AND METHODS
Various forms of
the Asian part of the range were sampled from the larg-
est rivers and water basins and thus represent the entire
Kamchatka geographical group (Table 1, Fig. 1). Since
this species is represented by various epigenetic vari-
ants , the river (resident) and anadromous (typically
anadromous) forms were included in the material. The
form attribution was determined by scale analysis and
by determining the Sr/Ca content in the otoliths .
The origin of the scarcely studied Shantarian
remains unknown. This is the only island population on
the Asian part of the range.
Our study was conducted in two stages. First, we
aimed to assess total variation and divergence of the
mitochondrial genome. For this purpose, restric-
tion analysis was performed using a set of restriction
endonucleases widely applied to the salmon group.
Using this method, a large region of mtDNA (at least
14%) was studied, which included control gene region
and the cytochrome b gene. The latter was afterwards
studied by sequencing.
was performed on the samples
from four rivers of Western and Eastern
coast of Kamchatka (Zhupanova, Krutogorovo, Voyam-
polka, and Tigil’) (Fig. 1, Table 1). These are large, geo-
graphically remote river systems.
from these rivers differ in population structures and
speciﬁc genetics at the level of protein polymorphism
. The samples selected for the river phenotype were
Total DNA was isolated from the frozen muscle tis-
sue by a standard method including cell lysis by 3% Na
sarcosyl, lysate incubation with proteinase K (100
for 3 h at 60
C, and deproteinization with mixtures phe-
nol–chloroform (1 : 1) and chloroform–isoamyl alcohol
Genetic Divergence of Mykizha
) from Kamchatka
Inferred from Restriction Analysis and Sequencing
of mtDNA Cytochrome b Gene
S. D. Pavlov
, A. A. Kolesnikov
, M. N. Melnikova
, and M. V. Ushakova
Department of Ichthyology and Molecular Biology, Moscow State University, Moscow, 119899 Russia;
Belozersky Research Institute of Physicochemical Biology, Moscow State University, Moscow, 119992 Russia
Received March 19, 2004
—The populations of mykizha
from western and eastern coasts of Kamchatka
were studied by restriction analysis of a fragment of ﬁsh mitochondrial genome that included the control region
and the region of the cytochrome b gene (
). The restriction patterns obtained with ﬁve enzymes (
I) were identical in all studied individuals. Sequencing of the
gene showed high
similarity between all samples (99.6–100%). In general, the geographical group of
from Kamchatka is
monophyletic with low genetic divergence at the population level. Shantarian
originates most likely
from that native to Kamchatka.