Genetic differentiation of Altai-Sayan endemic Hedysarum theinum Krasnob. (Fabaceae) evaluated by inter-simple sequence repeat analysis

Genetic differentiation of Altai-Sayan endemic Hedysarum theinum Krasnob. (Fabaceae) evaluated by... The objective of the research described in this paper was to evaluate the level of genetic variability and differentiation between the populations of Hedysarum theinum Krasnob., endemic species of Altai-Sayan Mountains. In this study, six inter-simple sequence repeat (ISSR) primers were used and generated a total of 132 molecular markers, 126 of which were polymorphic (95.5%). The large amount of DNA polymorphism at the intrapopulation level (H pop/H sp = 70.5) was evidenced by the analysis of a molecular variance (AMOVA) of 88.2%. The high genetic similarity (I = 0.875) detected between the populations of H. theinum reflects the strongly restricted endemic range of H. theinum, which facilitates the gene flow among populations and outcrossing. Due to the significant genetic diversity revealed among individuals against a background of moderate population differentiation collecting samples for ex situ H. theinum conservation from a small number of populations is acceptable. Finally, selected highly polymorphic ISSR markers produced consistently repeatable patterns are discussed as a powerful tool for genotype identification and the qualification of root feedstock. Russian Journal of Genetics Springer Journals

Genetic differentiation of Altai-Sayan endemic Hedysarum theinum Krasnob. (Fabaceae) evaluated by inter-simple sequence repeat analysis

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Springer US
Copyright © 2013 by Pleiades Publishing, Inc.
Biomedicine; Human Genetics; Animal Genetics and Genomics; Microbial Genetics and Genomics
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