1022-7954/05/4109- © 2005 Pleiades Publishing, Inc.
Russian Journal of Genetics, Vol. 41, No. 9, 2005, pp. 1028–1034. Translated from Genetika, Vol. 41, No. 9, 2005, pp. 1251–1258.
Original Russian Text Copyright © 2005 by Karpinskaya, Buzovkina.
In this study, we have examined a character related
to complex disturbances of shoot morphogenesis in
radish, in order to ﬁnd genes controlling plant growth
and development. We used a genetic collection of
inbred radish lines. The advantages of this collection
are a high level of inbreeding and a great number of
lines with morphological defects, which, according to
preliminary data, are hormonal mutants for all major
groups of phytohormones.
The character studied, termed by us
(Fig. 1), is characteristic of two lines from this genetic
collection, 16 and 26, which are of different origin. At
the present stage of the work, we have performed
hybrid analysis of the wavy shoots character in radish
and have studied its joint inheritance with marker char-
MATERIALS AND METHODS
We used lines of generation 36–39 from the genetic
collection of radish
Research Institute, St. Petersburg State University) and
their ﬁrst- and second-generation hybrids. The lines
differed in a number of morphological, physiological,
and biochemical characters (Table 1) [1–4]. The char-
acter of wavy shoots is characteristic of lines 16 and 26.
The plants were grown during 7 to 8 months in a
greenhouse with subsequent planting in the ﬁeld. The
wavy shoots character was analyzed as it appeared in
plant ontogeny. At the ﬁrst stage of the work, we
assessed the manifestation of the morphological distur-
bance, expressed as wavy shoots, in scores:
0: normal development of shoots of the ﬁrst and sec-
ond orders, bends do not exceed 10
1: wavy bends of shoots of the second and third
order up to 60
2: more pronounced bends of shoots of the second
and third order up to 90
3: bends are observed on shoots of the ﬁrst to third
order, reaching 180
4: bends are observed on shoots of the ﬁrst to third
order, reaching 270
Forms scored at 1 to 4 were regarded as having the
Biometrical analysis of the results was conducted
test and its partition into components. The
strength of linkage was estimated using the RECOMB
Genetic Control of the Wavy Shoots Character
L. I. Karpinskaya and I. S. Buzovkina
Department of Genetics and Selection, St. Petersburg State University, St. Petersburg, 199034 Russia;
fax: (812) 328-05-41; e-mail: firstname.lastname@example.org
Received September 29, 2004
—The results of genetic analysis of the wavy shoots character supported the hypothesis on the exist-
ence of two genes,
, whose mutations caused wavy shoots in radish lines 16 and 26, respectively.
As shown by analysis of joint inheritance with marker characters, wavy shoots were inherited independently of
the biochemical markers (genes
Lap1, Ep, Dia1
was shown to be linked to genes
; the mode of inheritance depended on the cross combination. In hybrids with mutant line 16, plants with
novel morphological defects, which were absent in the parental forms, were detected. Possible mechanisms of
new morphological abnormalities and change of linkage in the combinations analyzed are discussed.
Character of wavy shoots in radish. Left: a plant of the
mutant phenotype (line 16); right: normal plant (line 26).