1022-7954/02/3804- $27.00 © 2002
Russian Journal of Genetics, Vol. 38, No. 4, 2002, pp. 399–407. Translated from Genetika, Vol. 38, No. 4, 2002, pp. 497–506.
Original Russian Text Copyright © 2002 by Solodukhina.
Leaf rust, caused by
among the most harmful rye diseases. It causes a 21–
39% loss of short-stalked rye yield [1, 2]. Sometimes,
an early epiphytotic of leaf rust can reduce the yield of
short-stalked rye by 60–80% .
Information on the genetic control of resistance to
leaf rust in rye is scarce and fragmentary. Most studies
have been conﬁned to the mode of inheritance of this
trait. It was found that race-speciﬁc resistance to leaf
rust is controlled, as a rule, by independent dominant
Populations of some rye varieties contain genotypes
resistant to particular leaf rust isolates [12–15]. Cana-
dian researchers discovered monogenic, digenic, and
trigenic control of resistance to some clones of the
pathogen in self-fertile rye lines [16, 17].
Previously, we determined the inheritance of ﬁeld
resistance to a leaf rust population in lines obtained
with the use of resistant plants found in populations of
rye varieties. The monogenic control of the trait was found
in 37 accessions and digenic, in 4 accessions [18, 19].
The identity of the oligogenes controlling leaf rust
resistance in rye was ﬁrst investigated at the All-Russia
Vavilov Institute of Plant Industry (VIR). The method
proposed by us  and based on test crosses of dihet-
erozygotes for genes under study was used to determine
the allelic relationships among dominant genes.
It was found that leaf rust resistance in the acces-
sions Sanim, Malysh 72-2, Chulpan 3, and Novozybk-
ovskaya 4-2 is controlled by independent dominant oli-
gogenes designated as
Lr4, Lr5, Lr6
tively [18–20]. The designations
were formerly assigned to genes of wheat substitution
lines during investigation of leaf rust resistance in
wheat and rye [21, 22].
Studies on localization of genes
trolling the resistance in local rye landraces were
launched at the Institute of Crops (Gross Lusewitz,
was found to be linked with the
gene controlling the synthesis of the isoenzyme Aco1
(recombination frequency 0.02
was localized on chromosome 6R of rye. Linkage
and genetic RAPD markers OPO-07
and OPY-11 was established with recombination fre-
quencies of 0.16
0.117% and 0.09
tively. The chromosomal localization of this gene has
not been determined [23–25].
In addition to test crosses, the method of test clones
is widely applied for determining the identity of genes
controlling the resistance to obligate phytopathogens.
This method involves infection of specimens possess-
ing known resistance genes and specimens under study
with pathogen clones of tagged virulence . This
method is based on Flohr’s gene-for-gene postulate,
which states that a speciﬁc gene for pathogen virulence
corresponds to each host resistance gene . If at least
one of the resistance genes is opposed by an avirulence
allele, a resistance response occurs. We do not know
any studies in which this approach would be used to
determine the identity of genes controlling the resis-
tance of rye to
The purpose of the present study is to identify the
genes in 11 donors of leaf rust resistance in rye.
Genetic Characterization of Rye Accessions
with Regard to Leaf Rust Resistance
O. V. Solodukhina
All-Russia Vavilov Institute of Plant Industry, St. Petersburg, 189620 Russia
Received March 22, 2001; in ﬁnal form, July 11, 2001
—The genetic control of leaf rust resistance has been investigated by test crosses and the test-clone
method in the rye accessions Malysh 72-2, Chulpan 3, Immunnaya 4, Yaroslavna 3, Lovashpatonae 2, Gotor 2,
Talovskaya 12-2, Braunrostresisten 2, Orlovskaya 9-2, 3098/18, and 4001/10. It has been found that this trait
is controlled by a set of nonidentical oligogenes. Dominant gene
controls leaf rust resistance in Chulpan 3
and Immunnaya 4;
, in Malysh 72-2; and
, in Yaroslavna 3. Test crosses have shown that genes
differ from the genes determining the resistance in Gotor 2 and Braunrostresisten 2. In German accessions
4001/10 and 3098/18, the resistance to the Petersburg population of the pathogen is controlled by nonallelic
It has been found that gene
is not identical to the resistance gene of Lovashpatonae 2,
is not identical to
In all accessions, resistance to the leaf rust population is controlled by dominant
genes from the stage of seedlings till maturity. Highly efﬁcient
genes are present in the accessions Malysh 72-2,
Chulpan 3, Immunnaya 4, Yaroslavna 3, Lovashpatonae 2, Gotor 2, Talovskaya 12-2, 3098/18, and 4001/10. In
addition to the oligogenes, most accessions possess genes efﬁciently controlling particular clones of the pathogen.