Genetic characterization of porcine epidemic diarrhea virus in Korea from 1998 to 2013

Genetic characterization of porcine epidemic diarrhea virus in Korea from 1998 to 2013 The number of porcine epidemic diarrhea (PED) cases has increased over the past 20 years in Korea, with a major outbreak in 2013. A total of 27 Korean strains from 1998 to 2013 were analyzed (excluding the noncoding regions) and divided into two groups for comparison of the spike (S), ORF3, envelope (E), membrane (M), and nucleocapsid (N) genes with those of reference strains, vaccine strains, and previously identified strains based on phylogenetic analysis. Analysis of the selection patterns of PEDV isolated in Korea indicated positive selection of nine nonsynonymous sites in the S and N proteins and negative selection at 97 sites for all of the proteins. Interestingly, eight nonsynonymous mutations in S showed no significant pattern change over the 15-year period, and one of eight mutation sites was found only in IC05TK, GN05DJ, and KNU0802 in the epidemic years 2005 and 2008. These eight mutations were also present during the epidemic years in China. Furthermore, of the signs of positive selection in the S protein, the conservative substitutions were more frequent than radical substitutions in PEDVs, suggesting that the evolution of Korean strains has been slow. Serological cross-reactivity was detected between three field PEDVs and two vaccine strains, with different serum neutralization titers. In conclusion, although Korean PEDVs have been evolving slowly, their diverse antigenicity and genetics imply that multilateral efforts to prevent future PED outbreaks are required. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Genetic characterization of porcine epidemic diarrhea virus in Korea from 1998 to 2013

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Publisher
Springer Vienna
Copyright
Copyright © 2015 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-015-2353-y
Publisher site
See Article on Publisher Site

Abstract

The number of porcine epidemic diarrhea (PED) cases has increased over the past 20 years in Korea, with a major outbreak in 2013. A total of 27 Korean strains from 1998 to 2013 were analyzed (excluding the noncoding regions) and divided into two groups for comparison of the spike (S), ORF3, envelope (E), membrane (M), and nucleocapsid (N) genes with those of reference strains, vaccine strains, and previously identified strains based on phylogenetic analysis. Analysis of the selection patterns of PEDV isolated in Korea indicated positive selection of nine nonsynonymous sites in the S and N proteins and negative selection at 97 sites for all of the proteins. Interestingly, eight nonsynonymous mutations in S showed no significant pattern change over the 15-year period, and one of eight mutation sites was found only in IC05TK, GN05DJ, and KNU0802 in the epidemic years 2005 and 2008. These eight mutations were also present during the epidemic years in China. Furthermore, of the signs of positive selection in the S protein, the conservative substitutions were more frequent than radical substitutions in PEDVs, suggesting that the evolution of Korean strains has been slow. Serological cross-reactivity was detected between three field PEDVs and two vaccine strains, with different serum neutralization titers. In conclusion, although Korean PEDVs have been evolving slowly, their diverse antigenicity and genetics imply that multilateral efforts to prevent future PED outbreaks are required.

Journal

Archives of VirologySpringer Journals

Published: Apr 1, 2015

References

  • TGEV nucleocapsid protein induces cell cycle arrest and apoptosis through activation of p53 signaling
    Ding, L; Huang, Y; Du, Q; Dong, F; Zhao, X; Zhang, W; Xu, X; Tong, D
  • Phylogenetic analysis of porcine epidemic diarrhea virus field strains prevailing recently in China
    Gao, Y; Kou, Q; Ge, X; Zhou, L; Guo, X; Yang, H
  • Multiplex real-time RT-PCR for the simultaneous detection and quantification of transmissible gastroenteritis virus and porcine epidemic diarrhea virus
    Kim, SH; Kim, IJ; Pyo, HM; Tark, DS; Song, JY; Hyun, BH
  • Completion of the porcine epidemic diarrhoea coronavirus (PEDV) genome sequence
    Kocherhans, R; Bridgen, A; Ackermann, M; Tobler, K
  • Isolation of porcine epidemic diarrhea virus (PEDV) in Korea
    Kweon, CH; Kwon, BJ; Jung, TS; Kee, YJ; Hur, DH; Hwang, EK; Rhee, JC; An, SH

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