ISSN 10227954, Russian Journal of Genetics, 2012, Vol. 48, No. 6, pp. 587–591. © Pleiades Publishing, Inc., 2012.
Original Russian Text © V.P. Perevozkin, A.A. Printseva, P.V. Maslennikov, S.S. Bondarchuk, 2012, published in Genetika, 2012, Vol. 48, No. 6, pp. 692–697.
Acoustic signaling is widespread among insects,
primarily flying ones. Acoustic features play an impor
tant role in reproduction, allowing individuals of dif
ferent sexes to meet and contributing to the reproduc
tive isolation of closely related sympatric species .
Acoustic communication of dipterous insects is of par
ticular interest because it is necessary to better under
stand the complex forms of their living activity, to reg
ulate their behavior, and to control the population size
in vectors of serious transmissive diseases.
Malaria mosquitoes of the genus
(Diptera, Culicidae) attract particular attention in
biology and medicine in view of their epidemiological
significance. At the same time, species of the genus
provide a convenient model for genetic and ecological
analyses [2, 3]. Cytogenetic studies of Palearctic
malaria mosquitoes revealed several sibling species,
which were combined in the
species complex; some of the species are characterized
by intraspecific inversion polymorphism, which has
adaptive significance .
In spite of the interest in malaria mosquitoes, only
few works focused on their acoustics, and the mecha
nisms of their acoustic communication are poorly
understood. Investigation of the acoustic characteris
tics in malaria mosquitoes may help to develop effi
cient means for regulating their population sizes.
Thus, information on the acoustics of mosquitoes is of
both theoretical and applied significance.
The objective of this work was to study the acoustic
mechanisms of sexual behavior in species and within
species groups of malaria mosquitoes belonging to the
We determined the acoustic characteristics of mos
quitoes of different sexes in two model species,
; compared the sound frequen
cies between intraspecific karyotypic forms of
; and studied the possible relationship between
acoustic characteristics and preferences in selecting
the sexual partner.
MATERIALS AND METHODS
We examined adult mosquitoes of two species,
(progenies of females captured in nature)
(a laboratory strain). Gonoactive
females were collected from a cowshed in a
village of Tegul’det, Tomsk oblast on August 4, 2010.
The females were transported to Tomsk and, on the
next day, were individually placed in separate vials
filled with water to onefourth of the volume for ovi
position. After eclosion, firstinstar larvae were trans
ferred into plastic dishes with 200 mL of tap water,
which was allowed to stand preliminarily. The larvae
were fed a special dry mixture up to pupation. The air
temperature was 20–24°C during development. The
line was maintained in the same condi
tions on the same diet.
At the fourth instar, five larvae were selected from
progeny and were fixed with alcohol–
Genetic Aspects of Sexual Behavior in Malaria Mosquitoes
on the Basis of Specific Acoustic Signals at Mating
V. P. Perevozkin
, A. A. Printseva
, P. V. Maslennikov
, and S. S. Bondarchuk
Institute of Biology and Biophysics, Tomsk State University, Tomsk, 634050 Russia;
Department of General Biology and Ecology, Tomsk State Pedagogical University, Tomsk, 634061 Russia
Received November 10, 2011
—Acoustic characteristics were studied in two species of the
The species were found to clearly differ in sound frequencies, which was
assumed to play a key role in species identification during mating in regions of their sympatric distribution.
The sound spectrum in
was far more diverse than in
, which was associated with
intraspecific inversion polymorphism of the former. Mosquitoes with the inversion combinations that were
most common in populations of the central region of the
species area specifically differed in acous
tic signal spectrum from each other. Hence, sound communication within the species was considered to be
the main mechanism that is responsible for sexual partner selection and determines the chromosome associ
ations observed earlier in individual karyotypes. Since males carrying different inversion combinations signif
icantly differed in acoustic characteristics, females were assumed to play a main role in selecting the sexual