Genetic Analysis of Spontaneous Suppressors of the pho85 Mutation in Yeast Saccharomyces cerevisiae

Genetic Analysis of Spontaneous Suppressors of the pho85 Mutation in Yeast Saccharomyces cerevisiae Chaperones are known to play an important role in complexation of cyclin-dependent kinases with cyclins. In yeast cells growing in the presence of phosphate, cyclin-dependent kinase Pho85p and cyclin Pho80p form a complex and phosphorylate activator Pho4p. As a result, Pho4p is exported from the nucleus, and the PHO5 gene is not transcribed. The mutations suppressing thepho85 mutation were analyzed in order to identify genes which code for chaperones involved in the formation of the Pho80p–Pho85p complex in the presence of environmental phosphate. Dominant mutations DSP1, DSP2, and DSP4–6 were found. It is shown that the DSP1gene is 2.1 cM away from thePHO85 gene on chromosome XVI and probably coincides with the EGD1 gene coding for a chaperone. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Genetics Springer Journals

Genetic Analysis of Spontaneous Suppressors of the pho85 Mutation in Yeast Saccharomyces cerevisiae

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Publisher
Springer Journals
Copyright
Copyright © 2003 by MAIK “Nauka/Interperiodica”
Subject
Biomedicine; Human Genetics
ISSN
1022-7954
eISSN
1608-3369
D.O.I.
10.1023/A:1022058408193
Publisher site
See Article on Publisher Site

Abstract

Chaperones are known to play an important role in complexation of cyclin-dependent kinases with cyclins. In yeast cells growing in the presence of phosphate, cyclin-dependent kinase Pho85p and cyclin Pho80p form a complex and phosphorylate activator Pho4p. As a result, Pho4p is exported from the nucleus, and the PHO5 gene is not transcribed. The mutations suppressing thepho85 mutation were analyzed in order to identify genes which code for chaperones involved in the formation of the Pho80p–Pho85p complex in the presence of environmental phosphate. Dominant mutations DSP1, DSP2, and DSP4–6 were found. It is shown that the DSP1gene is 2.1 cM away from thePHO85 gene on chromosome XVI and probably coincides with the EGD1 gene coding for a chaperone.

Journal

Russian Journal of GeneticsSpringer Journals

Published: Oct 7, 2004

References

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