Generation of recombinant Norovirus-like particles (VLP) in the human endothelial kidney cell line 293T

Generation of recombinant Norovirus-like particles (VLP) in the human endothelial kidney cell... Noroviruses (NVs) are the major cause of non-bacterial outbreaks of gastroenteritis. Here we report a new alternative system to generate recombinant NV virus-like particles (rNV-VLP) in a human endothelial kidney cell line (HEK). Transfecting HEK-293T cells with an expression vector coding for the ORF-2 gene lead to the expression of the viral structural protein VP1 which spontaneously assembled into virus-like particles (VLP), as shown by electron microscopy. The transfected cells did not show a cytopathic effect and released rNV-VLP into the culture medium. The HEK-293T cell derived particles were morphologically indistinguishable to the rNV-VLP produced from baculovirus and the Venezuelan equine encephalitis virus (VEE)-replicon. The produced particles were stable for at least 2.5 months at 4 °C. Archives of Virology Springer Journals

Generation of recombinant Norovirus-like particles (VLP) in the human endothelial kidney cell line 293T

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Copyright © 2005 by Springer-Verlag/Wien
Biomedicine; Medical Microbiology; Virology; Infectious Diseases
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