Generation and characterization of potential dengue vaccine candidates based on domain III of the envelope protein and the capsid protein of the four serotypes of dengue virus

Generation and characterization of potential dengue vaccine candidates based on domain III of the... Dengue is currently one of the most important arthropod-borne diseases, causing up to 25,000 deaths annually. There is currently no vaccine to prevent dengue virus infection, which needs a tetravalent vaccine approach. In this work, we describe the cloning and expression in Escherichia coli of envelope domain III-capsid chimeric proteins (DIIIC) of the four dengue serotypes as a tetravalent dengue vaccine candidate that is potentially able to generate humoral and cellular immunity. The recombinant proteins were purified to more than 85 % purity and were recognized by anti-dengue mouse and human sera. Mass spectrometry analysis verified the identity of the proteins and the correct formation of the intracatenary disulfide bond in the domain III region. The chimeric DIIIC proteins were also serotype-specific, and in the presence of oligonucleotides, they formed aggregates that were visible by electron microscopy. These results support the future use of DIIIC recombinant chimeric proteins in preclinical studies in mice for assessing their immunogenicity and efficacy. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Generation and characterization of potential dengue vaccine candidates based on domain III of the envelope protein and the capsid protein of the four serotypes of dengue virus

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Publisher
Springer Vienna
Copyright
Copyright © 2014 by Springer-Verlag Wien
Subject
Biomedicine; Virology; Medical Microbiology; Infectious Diseases
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s00705-013-1956-4
Publisher site
See Article on Publisher Site

Abstract

Dengue is currently one of the most important arthropod-borne diseases, causing up to 25,000 deaths annually. There is currently no vaccine to prevent dengue virus infection, which needs a tetravalent vaccine approach. In this work, we describe the cloning and expression in Escherichia coli of envelope domain III-capsid chimeric proteins (DIIIC) of the four dengue serotypes as a tetravalent dengue vaccine candidate that is potentially able to generate humoral and cellular immunity. The recombinant proteins were purified to more than 85 % purity and were recognized by anti-dengue mouse and human sera. Mass spectrometry analysis verified the identity of the proteins and the correct formation of the intracatenary disulfide bond in the domain III region. The chimeric DIIIC proteins were also serotype-specific, and in the presence of oligonucleotides, they formed aggregates that were visible by electron microscopy. These results support the future use of DIIIC recombinant chimeric proteins in preclinical studies in mice for assessing their immunogenicity and efficacy.

Journal

Archives of VirologySpringer Journals

Published: Jul 1, 2014

References

  • A dengue-2 Envelope fragment inserted within the structure of the P64k meningococcal protein carrier enables a functional immune response against the virus in mice
    Hermida, L; Rodriguez, R; Lazo, L; Silva, R; Zulueta, A; Chinea, G; Lopez, C; Guzman, MG; Guillen, G
  • The fusion site of envelope fragments from each serotype of Dengue virus in the P64k protein, influence some parameters of the resulting chimeric constructs
    Zulueta, A; Hermida, L; Lazo, L; Valdes, I; Rodriguez, R; Lopez, C; Silva, R; Rosario, D; Martin, J; Guzman, MG; Guillen, G
  • Purified and highly aggregated chimeric protein DIIIC-2 induces a functional immune response in mice against dengue 2 virus
    Marcos, E; Gil, L; Lazo, L; Izquierdo, A; Brown, E; Suzarte, E; Valdes, I; Garcia, A; Mendez, L; Guzman, MG; Guillen, G; Hermida, L
  • Dengue-4 envelope domain III fused twice within the meningococcal P64k protein carrier induces partial protection in mice
    Lazo, L; Zulueta, A; Hermida, L; Blanco, A; Sanchez, J; Valdes, I; Gil, L; Lopez, C; Romero, Y; Guzman, MG; Guillen, G
  • Characterization of the interaction of domain III of the envelope protein of dengue virus with putative receptors from CHO cells
    Huerta, V; Chinea, G; Fleitas, N; Sarria, M; Sanchez, J; Toledo, P; Padron, G

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