Generation and Analysis of Soybean Plastid Transformants Expressing Bacillusthuringiensis Cry1Ab Protoxin

Generation and Analysis of Soybean Plastid Transformants Expressing Bacillusthuringiensis Cry1Ab... We describe the generation of fertile and homoplasmic soybean plastid transformants, expressing the Bacillus thuringiensis insecticidal protoxin Cry1Ab. Transgenes were targeted in the intergenic region of Glycine max plastome, between the rps12/7 and trnV genes and selection was carried out using the aadA gene encoding spectinomycin resistance. Molecular analysis confirmed the integration of the cry1Ab and aadA expression cassettes at the expected location in the soybean plastome, and the transmission of the transgenes to the next generation. Western blot analyses showed that the Cry1Ab protoxin is highly expressed in leaves, stems and seeds, but not in roots. Its expression confers strong insecticidal activity to the generated transgenic soybean, as exemplified with velvetbean caterpillar (Anticarsia  gemmatalis) Plant Molecular Biology Springer Journals

Generation and Analysis of Soybean Plastid Transformants Expressing Bacillusthuringiensis Cry1Ab Protoxin

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Kluwer Academic Publishers
Copyright © 2005 by Springer
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
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