Genbank accession number: AJ225087

Genbank accession number: AJ225087 Plant Molecular Biology 37: 1089, 1998. © 1998 Kluwer Academic Publishers. Printed in Belgium. GenBank accession #: AJ225087 1;2 1 1 Authors: Bhullar SS , Willmitzer L , Kossmann J Address: Max-Planck Institüt für Molekulare Pflanzenphysiologie, Karl-Liebknecht-Strasse 25, Haus 20, D-14476 Golm, Germany; Department of Biotechnology, Guru Nanak Dev University, Amritsar 143 005, PB, India. Source of sequence: A full-length clone (pK1W2) was isolated from a leaf-specific ZAP II-cDNA library of Vigna unguiculata (Cv. C-88). About 1  10 pfu of the ZAP II library were in mass in vivo excised to generate pBluescript SK(−) derivates which were screened by complementation of E. coli mutant strain KV 832. Trivial name: -amylase from cowpea (Vigna unguiculata). 0 0 Description: The pK1W2 clone is 1774 bp long with 23 bp 5 -UTR and a 240 bp 3 -UTR. The poly(A) tail starts at bp 1755. The clone has an ORF of 1491 bp. The predicted amino acid sequences of -amylases from different plant sources appear to be well conserved. The predicted amino acid sequence of - amylase from cowpea has very high homology (>90% similarity) with -amylases from other legumes and more than 77% similarity with -amylases from other plant sources (Table 1). Table 1. Amino acid similarity between beta-amylase from cowpea (Vigna unguiculata) and other plant sources. The predicted amino acid sequences for different -amylases were compared by BESTFIT method of Wisconsin Genetics Computer Group (Madison, WI; GCG Package, Version 8). The accession numbers are: Vigna unguiculata (EMBL accession number AJ225087), Arabidopsis thaliana (Swiss-Prot P25853), Glycine max (GenBank M92090), Hordeum vulgare (DDBJ D21349), Ipomoea batatas (Swiss-Prot P10537), Medicago sativa (GenBank AF026217), Secale cereale (PIR: locus JX2248), Triticum aestivum (EMBL X98504), Trifolium repens (GenBank AF049098), and Zea mays (Swiss-Prot P55005). Plant Molecular Biology Springer Journals

Genbank accession number: AJ225087

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Kluwer Academic Publishers
Copyright © 1998 by Kluwer Academic Publishers
Life Sciences; Biochemistry, general; Plant Sciences; Plant Pathology
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