Plant Molecular Biology 37: 1089, 1998. © 1998 Kluwer Academic Publishers. Printed in Belgium. GenBank accession #: AJ225087 1;2 1 1 Authors: Bhullar SS , Willmitzer L , Kossmann J Address: Max-Planck Institüt für Molekulare Pﬂanzenphysiologie, Karl-Liebknecht-Strasse 25, Haus 20, D-14476 Golm, Germany; Department of Biotechnology, Guru Nanak Dev University, Amritsar 143 005, PB, India. Source of sequence: A full-length clone (pK1W2) was isolated from a leaf-speciﬁc ZAP II-cDNA library of Vigna unguiculata (Cv. C-88). About 1 10 pfu of the ZAP II library were in mass in vivo excised to generate pBluescript SK(−) derivates which were screened by complementation of E. coli mutant strain KV 832. Trivial name: -amylase from cowpea (Vigna unguiculata). 0 0 Description: The pK1W2 clone is 1774 bp long with 23 bp 5 -UTR and a 240 bp 3 -UTR. The poly(A) tail starts at bp 1755. The clone has an ORF of 1491 bp. The predicted amino acid sequences of -amylases from different plant sources appear to be well conserved. The predicted amino acid sequence of - amylase from cowpea has very high homology (>90% similarity) with -amylases from other legumes and more than 77% similarity with -amylases from other plant sources (Table 1). Table 1. Amino acid similarity between beta-amylase from cowpea (Vigna unguiculata) and other plant sources. The predicted amino acid sequences for different -amylases were compared by BESTFIT method of Wisconsin Genetics Computer Group (Madison, WI; GCG Package, Version 8). The accession numbers are: Vigna unguiculata (EMBL accession number AJ225087), Arabidopsis thaliana (Swiss-Prot P25853), Glycine max (GenBank M92090), Hordeum vulgare (DDBJ D21349), Ipomoea batatas (Swiss-Prot P10537), Medicago sativa (GenBank AF026217), Secale cereale (PIR: locus JX2248), Triticum aestivum (EMBL X98504), Trifolium repens (GenBank AF049098), and Zea mays (Swiss-Prot P55005).
Plant Molecular Biology – Springer Journals
Published: Oct 6, 2004
It’s your single place to instantly
discover and read the research
that matters to you.
Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.
All for just $49/month
Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly
Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.
Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.
Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.
All the latest content is available, no embargo periods.
“Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”Daniel C.
“Whoa! It’s like Spotify but for academic articles.”@Phil_Robichaud
“I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”@deepthiw
“My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”@JoseServera