Plant Molecular Biology 37: 1089, 1998.
© 1998 Kluwer Academic Publishers. Printed in Belgium.
• GenBank accession #: AJ225087
Authors: Bhullar SS
, Willmitzer L
, Kossmann J
Max-Planck Institüt für Molekulare Pﬂanzenphysiologie, Karl-Liebknecht-Strasse 25, Haus 20,
D-14476 Golm, Germany;
Department of Biotechnology, Guru Nanak Dev University, Amritsar 143
005, PB, India.
Source of sequence: A full-length clone (pK1W2) was isolated from a leaf-speciﬁc λZAP II-cDNA library
of Vigna unguiculata (Cv. C-88). About 1 ×10
pfu of the λZAP II library were in mass in vivo excised
to generate pBluescript SK(−) derivates which were screened by complementation of E. coli mutant
strain KV 832.
Trivial name: β -amylase from cowpea (Vigna unguiculata).
Description: The pK1W2 clone is 1774 bp long with 23 bp 5
-UTR and a 240 bp 3
-UTR. The poly(A) tail
starts at bp 1755. The clone has an ORF of 1491 bp. The predicted amino acid sequences of β-amylases
from different plant sources appear to be well conserved. The predicted amino acid sequence of β-
amylase from cowpea has very high homology (>90% similarity) with β-amylases from other legumes
and more than 77% similarity with β-amylases from other plant sources (Table 1).
Table 1. Amino acid similarity between beta-amylase from cowpea (Vigna unguiculata) and other plant sources. The predicted amino acid
sequences for different β-amylases were compared by BESTFIT method of Wisconsin Genetics Computer Group (Madison, WI; GCG Package,
Version 8). The accession numbers are: Vigna unguiculata (EMBL accession number AJ225087), Arabidopsis thaliana (Swiss-Prot P25853),
Glycine max (GenBank M92090), Hordeum vulgare (DDBJ D21349), Ipomoea batatas (Swiss-Prot P10537), Medicago sativa (GenBank
AF026217), Secale cereale (PIR: locus JX2248), Triticum aestivum (EMBL X98504), Trifolium repens (GenBank AF049098), and Zea mays