Plant Molecular Biology 37: 1085, 1998. © 1998 Kluwer Academic Publishers. Printed in Belgium. Sequence announcements Authors: Chou, W-M and Kutchan, TM Address: Laboratorium für Molekulare Biologie, Universität München, Karlstrasse 29, 80333 München, Germany Source of sequence: The cDNA was isolated from a ZAPII cDNA library prepared from mRNA isolated from cell suspension cultures of Berberis stolonifera (barberry) 3 days after subculture. The clone was sequenced in pBluescript SK obtained by in vivo excision of the phage clone. Trivial name: Calreticulin from Berberis stolonifera. Description: The calreticulin cDNA clone is 1547 bp long and encodes a protein of 416 amino acids. The amino acid sequence is 50% identical to calreticulins from the animal kingdom. The cDNA was expressed in Spodoptera frugiperda Sf9 cells and was demonstrated to bind calcium. Acknowledgements: This work was supported by SFB 369 of the Deutsche Forschungsgemeinschaft, Bonn and Fonds der Chemischen Industrie, Frankfurt. 1 100 Bst ...MAIAERRSRSHLALR..VRDRVSAEVFFEERFEDGWESKWVKSDWKRDENMAGEWNFTSGKWNGDANDKGIQTSEDYRFYAISAAFPEFSNKGKTLV Rco .....M NPK L LFL S..LLAIA NR K T Y P E D Npl MATQRR NPS LHLITVFSLLVAV S N R E K H E N Ath ...M KLNPKFI LILFA..LVVI I K KR K D T KH A N S E D Zma ..MAIRKGSSYAVAAL ALASVAA AG Q K E K H E EY D 101 200 Bst FQFSVKHEQKLDCGGGYMKLLSGDVDQKKFGGDTPYSIMFGPDICGYSTKKVHAILTKGETNHLIKKDVPCETDQLTHVYTFILRPDASYSILIDNVEKQ Rco SST NYND E LVI T Npl YND E T Ath D T YNG E V T Zma L V G S T DGK L I T E 201 300 Bst SGSVYTDWDILPPKQIKDPEAKKPEDWEDKEYIPDPEDKKPEGYDDIPKEITDPEAKKPEDWDDEEDGEWTAPTIPNPDYKGEWKPKKIKNPNFKGKWKA Rco T L L K DE P D A E P Y Npl L S L T S DE F D E D E P Y Ath T L S L A K S D T A P TD E N AY Zma T I EH K D P D E P Q YQ 301 400 Bst PMIDNPDFKDDPDIYVFPKLKYVGIELWQVKSGTMFDNVLICDDPDYAKKLAEETWGKNKDAEKAAFDEAEKKKEEEEAKDDPTESD.DEKPDEEGESDG Rco E Y N L N E Q E S AD A DD DADDTE Npl L L V L IV E AI Q E R S AA AD AE DD ADDD .D Ath E EL V SL VS E H R S A A AE EAEDDDNEGD Zma Y A DS I L II T AL TF H E D AKGGDDE D LEDE DD KAD Bst EGDDESKDIDNEE........DDEDVHDEL* Rco D G SDAA D........SA * Npl DA K ESK............. DEA * Ath DS N SEETK AEETKEAEETDAA * Zma DKAD DAE SKDS....... KQ * Figure 1. Alignment of the deduced amino acid sequences of calreticulin from Berberis stolonifera (Bst) with Ricinus communis (Rco), Nicotiana plumbaginifolia (Npl), Arabidopsis thaliana (Ath) and Zea mays (Zma).
Plant Molecular Biology – Springer Journals
Published: Oct 6, 2004
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