Plant Molecular Biology 40: 747, 1999. © 1999 Kluwer Academic Publishers. Printed in the Netherlands. Sequence announcement EMBL accession #: AJ131961 1; 1 2 2 Authors: Wu WL ,Hsiao IL ,Fu YM andChenWH Address: Department of Biology, Cheng-Kung University, Tainan, Taiwan 700 ( author for correspon- dence); Department of Horticulture, Taiwan Sugar Research Institute Tainan, Taiwan Source of sequence: Sequence was determined from a PCR product, which was ligated to the pPCR-Script Amp SK(C) cloning vector (Stratagene), from genomic DNA of Phalaenopsis violacea, a wild species of orchid. Trivial name:rDNA ITS of Phalaenopsis violacea. Description: ITS region of ribosomal DNA lying between 17S and 25S rRNA genes was cloned from a wild species, Phalaenopsis violacea, with 234 bp in ITS1, 161 bp in 5.8S rRNA gene and 262 bp in ITS2. Nucleotide sequence of ITS region in Phalaenopsis violacea shares 74.3%, 69.0% and 67.5% identity with Stenoglottis longifolia, Bonatea speciosa,and Nicotiana rustica, respectively. Acknowledgements: This research was granted by Taiwan Sugar Corporation (NSC 87-TSC-B-006-003) to W.L.W. Figure 1. Nucleotide sequence of ITS region of rDNA from Phalaenopsis violacea.
Plant Molecular Biology – Springer Journals
Published: Oct 19, 2004
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