Plant Molecular Biology 39: 639, 1999.
© 1999 Kluwer Academic Publishers. Printed in the Netherlands.
• GenBank accession #: AF096617, AF097359, AF097360 and AF097361
Authors: Alicchio R
Address: Department of Biology, University of Bologna, Via Selmi 3, 40126 Bologna, Italy. Phone 0039
051 354157; fax 0039 051 251208; e-mail Alicchio@Biblio.Cib.Unibo.It
Source of sequences: The cDNAs were obtained by RT-PCR of total mRNA isolated from leaves of
Avena sterilis subsp. ludoviciana (AF096617), A. agadiriana (AF097359), A. strigosa (AF097360)
and A. clauda (AF097361) by using as primers two 21 nucleotide sequences derived from conserved
regions of rbcS genes in rice and wheat (Alicchio et al., 1997, Plant Bisystems 131: 175–180). The
PCR fragments were cloned into pGem-T easy vector (Promega)
Trivial name: rbcS cDNAs from Avena sterilis subsp. ludoviciana, A. agadiriana, A. strigosa, A. clauda
Description: The rbcS oat cDNAs are 504 bp long and encode a peptide of 169 amino acids. The amino
acid sequences of the four species of Avena show 95–99% identity. The oat sequences are 88% identical
to rbcS from T. aestivum, 90% to rbcS from H. vulgare, 82% to rbcS from O. sativa and 72% to rbcS
from Z. mays.
Acknowledgements: I thank Dr E. Nebuloso of Plant Genome Lab (Enea, Rome). The work was supported
by MURST (Ministero per la Ricerca Scientiﬁca e Tecnologica).
Table 1. Amino acid sequence of rbcS cDNA of Avena ludoviciana aligned with the
sequences of other species of Avena and of Triticum, Hordeum, Oryza and Zea.Dotsde-
note identity to the A. ludoviciana sequence. Dashes denote gaps introduced to maximize