Plant Molecular Biology 39: 639, 1999. © 1999 Kluwer Academic Publishers. Printed in the Netherlands. Sequence announcement Authors: Alicchio R Address: Department of Biology, University of Bologna, Via Selmi 3, 40126 Bologna, Italy. Phone 0039 051 354157; fax 0039 051 251208; e-mail Alicchio@Biblio.Cib.Unibo.It Source of sequences: The cDNAs were obtained by RT-PCR of total mRNA isolated from leaves of Avena sterilis subsp. ludoviciana (AF096617), A. agadiriana (AF097359), A. strigosa (AF097360) and A. clauda (AF097361) by using as primers two 21 nucleotide sequences derived from conserved regions of rbcS genes in rice and wheat (Alicchio et al., 1997, Plant Bisystems 131: 175–180). The PCR fragments were cloned into pGem-T easy vector (Promega) Trivial name: rbcS cDNAs from Avena sterilis subsp. ludoviciana, A. agadiriana, A. strigosa, A. clauda Description: The rbcS oat cDNAs are 504 bp long and encode a peptide of 169 amino acids. The amino acid sequences of the four species of Avena show 95–99% identity. The oat sequences are 88% identical to rbcS from T. aestivum, 90% to rbcS from H. vulgare, 82% to rbcS from O. sativa and 72% to rbcS from Z. mays. Acknowledgements: I thank Dr E. Nebuloso of Plant Genome Lab (Enea, Rome). The work was supported by MURST (Ministero per la Ricerca Scientiﬁca e Tecnologica). Table 1. Amino acid sequence of rbcS cDNA of Avena ludoviciana aligned with the sequences of other species of Avena and of Triticum, Hordeum, Oryza and Zea.Dotsde- note identity to the A. ludoviciana sequence. Dashes denote gaps introduced to maximize sequence alignment.
Plant Molecular Biology – Springer Journals
Published: Sep 29, 2004
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