Functions of the CCCH type zinc finger protein OsGZF1 in regulation of the seed storage protein GluB-1 from rice

Functions of the CCCH type zinc finger protein OsGZF1 in regulation of the seed storage protein... Glutelins are the most abundant storage proteins in rice grain and can make up to 80 % of total protein content. The promoter region of GluB-1, one of the glutelin genes in rice, has been intensively used as a model to understand regulation of seed-storage protein accumulation. In this study, we describe a zinc finger gene of the Cys3His1 (CCCH or C3H) class, named OsGZF1, which was identified in a yeast one-hybrid screening using the core promoter region of GluB-1 as bait and cDNA expression libraries prepared from developing rice panicles and grains as prey. The OsGZF1 protein binds specifically to the bait sequence in yeast and this interaction was confirmed in vitro. OsGZF1 is predominantly expressed in a confined domain surrounding the scutellum of the developing embryo and is localised in the nucleus. Transient expression experiments demonstrated that OsGZF1 can down-regulate a GluB-1-GUS (β-glucuronidase) reporter and OsGZF1 was also able to significantly reduce activation conferred by RISBZ1 which is a known strong GluB-1 activator. Furthermore, down-regulation of OsGZF1 by an RNAi approach increased grain nitrogen concentration. We propose that OsGZF1 has a function in regulating the GluB-1 promoter and controls accumulation of glutelins during grain development. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Functions of the CCCH type zinc finger protein OsGZF1 in regulation of the seed storage protein GluB-1 from rice

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Publisher
Springer Journals
Copyright
Copyright © 2013 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-013-0158-5
Publisher site
See Article on Publisher Site

Abstract

Glutelins are the most abundant storage proteins in rice grain and can make up to 80 % of total protein content. The promoter region of GluB-1, one of the glutelin genes in rice, has been intensively used as a model to understand regulation of seed-storage protein accumulation. In this study, we describe a zinc finger gene of the Cys3His1 (CCCH or C3H) class, named OsGZF1, which was identified in a yeast one-hybrid screening using the core promoter region of GluB-1 as bait and cDNA expression libraries prepared from developing rice panicles and grains as prey. The OsGZF1 protein binds specifically to the bait sequence in yeast and this interaction was confirmed in vitro. OsGZF1 is predominantly expressed in a confined domain surrounding the scutellum of the developing embryo and is localised in the nucleus. Transient expression experiments demonstrated that OsGZF1 can down-regulate a GluB-1-GUS (β-glucuronidase) reporter and OsGZF1 was also able to significantly reduce activation conferred by RISBZ1 which is a known strong GluB-1 activator. Furthermore, down-regulation of OsGZF1 by an RNAi approach increased grain nitrogen concentration. We propose that OsGZF1 has a function in regulating the GluB-1 promoter and controls accumulation of glutelins during grain development.

Journal

Plant Molecular BiologySpringer Journals

Published: Nov 27, 2013

References

  • A genome-wide survey of HD-Zip genes in rice and analysis of drought-responsive family members
    Agalou, A; Purwantomo, S; Övernas, E; Johannesson, H; Zhu, X; Estiati, A; Kam, RJ; Engstrom, P; Slamet-Loedin, IH; Zhu, Z; Wang, M; Xiong, L; Meijer, AH; Ouwerkerk, PBF
  • A highly efficient transient protoplast system for analyzing defence gene expression and protein–protein interactions in rice
    Chen, S; Tao, L; Zeng, L; Vega-Sanchez, M; Wang, GL; Umemura, K
  • The developmentally regulated bZIP factor ROM1 modulates transcription from lectin and storage protein genes in bean embryos
    Chern, MS; Eiben, HG; Bustos, MM
  • Engineered GFP as a vital reporter in plants
    Chiu, W; Niwa, Y; Zeng, W; Hirano, T; Kobayashi, H; Sheen, J
  • A CCCH-type zinc finger nucleic acid-binding protein quantitatively confers resistance against rice bacterial blight disease
    Deng, H; Liu, H; Li, X; Xiao, J; Wang, S
  • Profiling the expression of genes controlling rice grain quality
    Duan, M; Sun, SS
  • Isolation and purification of functional total RNA from blue-grained wheat endosperm tissues containing high levels of starches and flavonoids
    Gao, J; Liu, J; Li, B; Li, Z

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