ISSN 0012-4966, Doklady Biological Sciences, 2018, Vol. 478, pp. 37–39. © Pleiades Publishing, Ltd., 2018.
Original Russian Text © G.F. Metallov, E.N. Ponomareva, M.N. Sorokina, V.A. Grigoryev, A.A. Korchunov, 2018, published in Doklady Akademii Nauk, 2018, Vol. 478, No.
6, pp. 727–729.
Functional Orientation of Biochemical Processes
in Female Beluga × Sterlet (Acipenser ruthenus Linnaeus,
1758 × Huso huso Linnaeus, 1758) Hybrids in the Reproductive Cycle
G. F. Metallov, E. N. Ponomareva, M. N. Sorokina*, V. A. Grigoryev, and A. A. Korchunov
Presented by Academician G.G. Matishov September 15, 2009
Received September 27, 2017
Abstract—The article reports data on the dynamics of blood parameters and parameters of protein, fat, and
water–salt metabolism in female starlet × beluga hybrids kept under artificial conditions in the period of
reproductive cycle. Gonad maturation in female hybrids in the artificial environment causes clear changes in
the biochemistry of blood and of urine.
Biochemical parameters of blood have been suc-
cessfully used for correction of orientation of the
reproductive cycle in animal breeding for a long time
. Techniques of hematological control of the matu-
ration process in fish, including acipenserids, kept
under artificial conditions is at the stage of experimen-
tal research [2, 3].
The decisive role in regulation of water-salt metab-
olism in animals is played by the kidney. It reacts to
processes of the reproductive cycles by changes of the
salt and water composition of the urine . Investiga-
tions of the water–salt metabolism in acipenserids
demonstrate that natural maturation of gonads causes
changes of regulation of metabolism of water and of
electrolytes [5, 6]. Hormonal stimulation of matura-
tion in acipenserids leads to retention of water in the
fish body and decrease in blood osmolarity. Matura-
tion of acipenserids leads to a parallel increase in
osmolarity (salinity) of urine. However, the use of
physiological parameters characterizing dynamics of
water–salt metabolism as indexes of maturity of fish is
almost never considered [3, 6].
The present study deals with variability of bio-
chemical parameters of blood and urine in female
sterlet × beluga (Acipenser ruthenus Linnaeus, 1758 ×
Huso huso Linnaeus, 1758) hybrids in the process of
the reproductive cycle.
The fish were kept in a separate installation with
closed water supply (ICW) under regulated hydro-
logic-hydrochemical conditions. To reveal the effect
on dynamics of metabolism, the spawners whose
reproductive cells are at the final stage of maturation
were exposed to a low temperature of 6°С for over two
weeks. In this period, the expenditure of accumulated
lipids becomes active and formation of gonads is com-
pleted. In spawners of acipenserids kept in ICW at a
permanently high temperature, constant feeding with
artificial feeds disturbs the fat metabolism, thus inter-
fering with the normal development of sex cells.
The functional state of female hybrids with differ-
ent degrees of gonad maturity was estimated by the
content in blood of hemoglobin, serum protein, cho-
lesterol, total lipid, β-lipoprotein, and erythrocyte
sedimentation rate (ESR). The concentration of
ocsmotically active substances was determined in
blood serum and urine.
Biochemical parameters of blood were determined
by certified techniques and a PLIVA-Lachema Diag-
nostika S.R.O. (Czech Republic) and Olveks Diag-
nosticum-Yug (Russia) sets of reagents [7–13]. The
osmolarities of urine and of blood serum were deter-
mined cryoscopically by means of an OSKR-1
osmometer (Biomer, Russia) with further transforma-
tion into salinity. The data were statistically processed
by Student’s t test, the differences were considered sig-
nificant at p ≤ 0.05.
Analysis of dynamics of physiological parameters
of blood of the female hybrids at different stages of
gametogenesis demonstrated a higher level of bio-
chemical parameters in the fish whose gonads were at
the stage of active maturation (Table 1). A similar ten-
dency was previously shown [2, 3, 14] in wild female
acipenserids migrating for spawning with gonads at
Southern Scientific Center, Russian Academy of Sciences,
Rostov-on-Don, 344006 Russia
* e-mail: email@example.com