Access the full text.
Sign up today, get DeepDyve free for 14 days.
(1996)
A jojoba
Christiane Hanke, F. Wolter, Jack Coleman, Gabriele Peterek, M. Frentzen (1995)
A plant acyltransferase involved in triacylglycerol biosynthesis complements an Escherichia coli sn-1-acylglycerol-3-phosphate acyltransferase mutant.European journal of biochemistry, 232 3
T. Thomas (1993)
Gene expression during plant embryogenesis and germination: an overview.The Plant cell, 5
S. Clemens (1997)
Isolation of a Brassica napus cDNA accession no. AF009563 encoding 3-ketoacyl-CoA synthase, a condensing enzyme involved in the biosynthesis of very long chain fatty acids in seeds (PGR97-125)Plant Physiology, 115
Jitao, Zou, Vesna Katavić, E., Michael Giblin, Dennis, L., Barton, Samuel, MacKenzie, Wilfred Keller, Xu, Hu, David Taylora (1997)
Modification of seed oil content and acyl composition in the brassicaceae by expression of a yeast sn-2 acyltransferase gene.The Plant cell, 9
R. Jefferson, T. Kavanagh, M. Bevan (1987)
GUS fusions: beta‐glucuronidase as a sensitive and versatile gene fusion marker in higher plants.The EMBO Journal, 6
F. Ausubel, R. Brent, R. Kingston, D. Moore, J. Seidman, Jennifer Smith, K. Struhl, G. Barry (1988)
Current Protocols in Molecular Biology (New York: Greene Publishing Associates and Wiley-Interscience). Host-Range Shuttle System for Gene Insertion into the Chromosomes of Gram-negative Bacteria., 71
(1964)
The inheritance of erucic acid content in rapeseed ( Brassica napus )
D. Post-Beittenmiller (1996)
BIOCHEMISTRY AND MOLECULAR BIOLOGY OF WAX PRODUCTION IN PLANTS.Annual review of plant physiology and plant molecular biology, 47
S.L. Dellaporta, J. Wood, J.B. Hicks (1983)
A plant DNA minipreparationPlant Mol. Biol. Rep., 1
Bao, Pollard, Ohlrogge (1998)
The biosynthesis of erucic acid in developing embryos of brassica rapaPlant physiology, 118 1
W.W Lühs, A. Voss, J. Han, A. Gräfin zu Münster, D. Weier, F.P. Wolter, M. Frentzen, W. Friedt (1999)
Genetics and Breeding for Crop Quality and Resistance (Developments in Plant Breeding
J. Venkateswari, S. Kanrar, P.B. Kirti, V.G. Malathi, V.L. Chopra (1999)
Molecular cloning and characterization of FATTY ACID ELONGATION1 (BjFAE1) gene ofBrassica junceaJ. Plant Biochem. Biotechnol., 8
(1994)
Stand und Perspektiven der Züchtung von Raps
D. Weier, C. Hanke, Andreas Eickelkamp, W. Lühs, J. Dettendorfer, Elena Schaffert, C. Möllers, W. Friedt, F. Wolter, M. Frentzen (1997)
Trierucoylglycerol Biosynthesis in Transgenic Plants of Rapeseed (Brassica napus L.)Fett-lipid, 99
(1998)
Lunaria annua): metabolic intermediates of very long chain acyl-CoA products and substrate specificity
E. Lim, J. Keller, E. Ralston, H. Dooner (1995)
Directed Tagging of the Arabidopsis FATTY ACID
(1999)
Molecular cloning and characterization
J. Sandhu, C. Webster, J. Gray (1998)
A/T-rich sequences act as quantitative enhancers of gene expression in transgenic tobacco and potato plantsPlant Molecular Biology, 37
A. Millar, L. Kunst (1997)
Very-long-chain fatty acid biosynthesis is controlled through the expression and specificity of the condensing enzyme.The Plant journal : for cell and molecular biology, 12 1
D. Dorrell, R. Downey (1964)
THE INHERITANCE OF ERUCIC ACID CONTENT IN RAPESEED (BRASSICA CAMPESTRIS)Canadian Journal of Plant Science, 44
W. Lühs, A. Voss, J. Han, A. Münster, D. Weier, F. Wolter, M. Frentzen, W. Friedt (1999)
Genetic modification of erucic acid biosynthesis in Brassica napus
P. Barret, R. Delourme, Michel Renard, F. Domergue, René Lessire, M. Delseny, T. Roscoe (1998)
A rapeseed FAE1 gene is linked to the E1 locus associated with variation in the content of erucic acidTheoretical and Applied Genetics, 96
S. Altschul, W. Gish, W. Miller, E. Myers, D. Lipman (1990)
Basic local alignment search tool.Journal of molecular biology, 215 3
(1998)
A rapeseed
D. Weier, W. Lühs, J. Dettendorfer, M. Frentzen (1998)
sn-1-Acylglycerol-3-phosphate acyltransferase of Escherichia coli causes insertion of cis-11 eicosenoic acid into the sn-2 position of transgenic rapeseed oilMolecular Breeding, 4
F. Domergue, S. Chevalier, X. Santarelli, C. Cassagne, R. Lessire (1999)
Evidence that oleoyl-CoA and ATP-dependent elongations coexist in rapeseed (Brassica napus L.).European journal of biochemistry, 263 2
B. Andersson, R. Holman (1974)
Pyrrolidides for mass spectrometric determination of the position of the double bond in monounsaturated fatty acidsLipids, 9
(1995)
OleoylCoA is not an immediate substrate for fatty acid elongation in developing seeds
(1988)
Binary vectors
Andrew Bobb, Maw-Shenq Chern, Mauricio Bustos (1997)
Conserved RY-repeats mediate transactivation of seed-specific promoters by the developmental regulator PvALF.Nucleic acids research, 25 3
A. Hloušek-Radojčić, H. Imai, J. Jaworski (1995)
Oleoyl‐CoA is not an immediate substrate for fatty acid elongation in developing seeds of Brassica napusPlant Journal, 8
H. Ochman, Anne Gerber, D. Hart (1988)
Genetic applications of an inverse polymerase chain reaction.Genetics, 120 3
(1972)
Plant breeding for improved yield and quality
K. Lardizabal, James Metz, Tetsuo Sakamoto, William Hutton, Michael Pollard, Michael Lassner (2000)
Purification of a jojoba embryo wax synthase, cloning of its cDNA, and production of high levels of wax in seeds of transgenic arabidopsis.Plant physiology, 122 3
M. Fourmann, P. Barret, Michel Renard, Georges Pelletier, R. Delourme, Dominique Brunel (1998)
The two genes homologous to Arabidopsis FAE1 co-segregate with the two loci governing erucic acid content in Brassica napusTheoretical and Applied Genetics, 96
(1991)
Acyl-CoA elongase from higher plant
B. Lööf, L. Appleqvist (1972)
Rapeseed
P. Sperling, U. Zähringer, E. Heinz (1998)
A Sphingolipid Desaturase from Higher PlantsThe Journal of Biological Chemistry, 273
(1999)
A G-box element from the Catharanthus roseustrictosidine synthase
F.M. Ausubel, R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, K. Struhl, L.M. Albright, D.M. Coen, A. Varki (1995)
Current Protocols in Molecular Biology
C. Brough, Jane Coventry, W. Christie, J. Kroon, Adrian Brown, T. Barsby, A. Slabas (1996)
Towards the genetic engineering of triacylglycerols of defined fatty acid composition: major changes in erucic acid content at the sn-2 position affected by the introduction of a 1-acyl-sn-glycerol-3-phosphate acyltransferase from Limnanthes douglasii into oil seed rapeMolecular Breeding, 2
(1998)
Tri - acylglycerol composition in Brassica napus is associated with variation in the sequence of the FAE 1 gene
J.L. Harwood (1980)
The Biochemistry of Plants
M. Lassner, C. Levering, H. Davies, D. Knutzon (1995)
Lysophosphatidic Acid Acyltransferase from Meadowfoam Mediates Insertion of Erucic Acid at the sn-2 Position of Triacylglycerol in Transgenic Rapeseed Oil, 109
Kjell Stålberg, M. Ellerstrom, Staffan Sjodahl, I. Ezcurra, P. Wycliffe, L. Rask (1998)
Heterologous and homologous transgenic expression directed by a 2S seed storage promoter of Brassica napusTransgenic Research, 7
A. Yephremov, E. Wisman, P. Huijser, Casper Huijser, Kirsten Wellesen, H. Saedler (1999)
Characterization of the FIDDLEHEAD Gene of Arabidopsis Reveals a Link between Adhesion Response and Cell Differentiation in the EpidermisPlant Cell, 11
E. Fehling, Kumar Mukherjee (1991)
Acyl-CoA elongase from a higher plant (Lunaria annua): metabolic intermediates of very-long-chain acyl-CoA products and substrate specificity.Biochimica et biophysica acta, 1082 3
B. Stefansson (1983)
6 – The Development of Improved Rapeseed Cultivars
D.W. James, E. Lim, J. Keller, I. Plooy, H.K. Dooner (1995)
Directed tagging of the Arabidopsis fatty acid elongation1 (FAE1) gene with the maize transposon activatorPlant Cell, 7
A. Millar, S. Clemens, S. Zachgo, E. Giblin, D. Taylor, L. Kunst (1999)
CUT1, an Arabidopsis Gene Required for Cuticular Wax Biosynthesis and Pollen Fertility, Encodes a Very-Long-Chain Fatty Acid Condensing EnzymePlant Cell, 11
M. Ellerström, Kjell Stålberg, I. Ezcurra, L. Rask (1996)
Functional dissection of a napin gene promoter: identification of promoter elements required for embryo and endosperm-specific transcriptionPlant Molecular Biology, 32
(1997)
Isolation of a Brassica napus cDNA encoding 3 - ketoacyl - CoA synthase , a condensing enzyme involved in the biosynthesis of very long chain fatty acids in seeds ( PGR 97 - 125 )
Michael Lassner, K. Lardizabal, James Metz (1996)
A jojoba beta-Ketoacyl-CoA synthase cDNA complements the canola fatty acid elongation mutation in transgenic plants.The Plant cell, 8
(1995)
A plant acyl-transferase involved in triacylglycerol biosynthesis
M. Block, D. Brouwer, P. Tenning (1989)
Transformation of Brassica napus and Brassica oleracea Using Agrobacterium tumefaciens and the Expression of the bar and neo Genes in the Transgenic Plants.Plant physiology, 91 2
Steven ScofieldS, Martha Crouch (1987)
Nucleotide sequence of a member of the napin storage protein family from Brassica napus.The Journal of biological chemistry, 262 25
P.M. von Wettstein-Knowles (1993)
Lipid Metabolism in Plants
J. Todd, D. Post-Beittenmiller, J. Jaworski (1999)
KCS1 encodes a fatty acid elongase 3-ketoacyl-CoA synthase affecting wax biosynthesis in Arabidopsis thaliana.The Plant journal : for cell and molecular biology, 17 2
(1995)
Directed tagging
J. Harwood (1980)
Plant Acyl Lipids: Structure, Distribution, and Analysis
G. An, P.R. Ebert, A. Mitra, S.B. Ha (1988)
Plant Molecular Biology Manual
E. Cahoon, E. Marillia, K. Stecca, S. Hall, D. Taylor, A. Kinney (2000)
Production of fatty acid components of meadowfoam oil in somatic soybean embryos.Plant physiology, 124 1
T. Roscoe, R. Lessire, M. Renard, M. Delseny (1998)
Advances in Plant Lipid Research
(1998)
ABrassica napuscDNA restores the deficiency of canola fatty acid elongation at a high level
P. Sperling, U. Zähringer, E. Heinz (1998)
A sphingolipid de-saturase from higher plants: Identification of a new cytochrome b5 fusion proteinJ. Biol. Chem., 273
R. Pruitt, Jean-Philippe Vielle-Calzada, S. Ploense, U. Grossniklaus, S. Lolle (2000)
FIDDLEHEAD, a gene required to suppress epidermal cell interactions in Arabidopsis, encodes a putative lipid biosynthetic enzyme.Proceedings of the National Academy of Sciences of the United States of America, 97 3
H. Bäumlein, Istvan Nagy, Raimundo Villarroel, Dirk Inzé, U. Wobus (1992)
Cis-analysis of a seed protein gene promoter: the conservative RY repeat CATGCATG within the legumin box is essential for tissue-specific expression of a legumin gene.The Plant journal : for cell and molecular biology, 2 2
P. Ouwerkerk, J. Memelink (1999)
A G-box element from the Catharanthus roseus strictosidine synthase (Str) gene promoter confers seed-specific expression in transgenic tobacco plantsMolecular and General Genetics MGG, 261
W. Lühs, W. Friedt (1994)
Stand und Perspektiven der Züchtung von Raps (Brassica napus L.) mit hohem Erucasäure-Gehalt im Öl für industrielle Nutzungszwecke†Fett-lipid, 96
A. Millar, M. Wrischer, L. Kunst (1998)
Accumulation of Very-Long-Chain Fatty Acids in Membrane Glycerolipids Is Associated with Dramatic Alterations in Plant MorphologyPlant Cell, 10
B. Stefansson, F. Hougen, R. Downey (1961)
NOTE ON THE ISOLATION OF RAPE PLANTS WITH SEED OIL FREE FROM ERUCIC ACIDCanadian Journal of Plant Science, 41
Seed-specifically expressed β-ketoacyl-CoA synthase genes of Brassica napus (Bn-FAE1.1 genes) were cloned from two cultivars, namely Askari, a high-erucic-acid type, and Drakkar, a low-erucic-acid type. The genes from the two cultivars were found to be nearly identical. They encode proteins of 507 amino acids, the sequences of which differ only at position 282. The Bn-FAE1.1 gene of Askari, unlike that of Drakkar, was functionally expressed in yeast cells suggesting that the single amino acid exchange effects the low erucic acid phenotype at the E1 gene locus. In yeast cells the β-ketoacyl-CoA synthase of Askari elongated not only oleoyl but also palmitoleoyl groups as well as saturated acyl groups in such a way that monounsaturated acyl groups of 22 carbons and saturated ones of 26 carbons were formed as main products. A reporter gene fused to the promoter region of the Bn-FAE1.1 gene from Askari showed seed-specific expression in transgenic rapeseed plants. Over-expression of the coding region of the Askari gene in developing seeds of transgenic Drakkar plants resulted in a significant increase in the levels of eicosenoic acid and erucic acid esterified in the seed oil. On the other hand, in transgenic high-erucic-acid rapeseed plants the increase in erucic acid level was at most 60% although the chimeric Bn-FAE1.1 gene was co-expressed with an erucoyl-CoA-specific lysophosphatidate acyltransferase gene enabling trierucoyl glycerol to accumulate in the seed oil.
Plant Molecular Biology – Springer Journals
Published: Oct 3, 2004
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.