Functional characterization of Arabidopsis Dof transcription factor AtDof4.1

Functional characterization of Arabidopsis Dof transcription factor AtDof4.1 The plant specific Dof (DNA Binding with One Zinc Finger) transcription factors (TFs) perform many different functions, and some members of this family appear to play an important role in the evolution of the vascular system characteristic of land plants. Here we report the functional characterization of a Dof TF from Arabidopsis (At4g00940-AtDof4.1). Transcriptional fusion with GUS or GFP reporter indicated that the AtDof4.1 strongly expresses in the young seedlings, particularly in the vascular tissues. The reporter expression was strictly restricted to the pericycle cells of the vascular tissues in the mature root zone and root cap while absent from the hypocotyls. The developmental phenotypes of its loss-of-function mutants were indistinguishable from those of the wild type probably due to the functional redundancy in the members of Arabidopsis Dof family. However, the overexpression lines showed severe growth retardation and delayed stem bolting and flowering. Similarly the overexpression of AtDof4.1 fusion with a super repressor domain (SRD) showed severely delayed flowering and defective reproductive organ development. In addition, AtDof4.1-SRD overexpression lines had the reduced size of the organs, such as leaves, flowers, and siliques. Due to similar morphology of overexpression AtDof4.1 and AtDof4.1-SRD lines, we suggest that AtDof4.1 functions as a transcriptional repressor. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Functional characterization of Arabidopsis Dof transcription factor AtDof4.1

Loading next page...
 
/lp/springer_journal/functional-characterization-of-arabidopsis-dof-transcription-factor-k40Tzy20j1
Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2013 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443712060027
Publisher site
See Article on Publisher Site

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

Monthly Plan

  • Read unlimited articles
  • Personalized recommendations
  • No expiration
  • Print 20 pages per month
  • 20% off on PDF purchases
  • Organize your research
  • Get updates on your journals and topic searches

$49/month

Start Free Trial

14-day Free Trial

Best Deal — 39% off

Annual Plan

  • All the features of the Professional Plan, but for 39% off!
  • Billed annually
  • No expiration
  • For the normal price of 10 articles elsewhere, you get one full year of unlimited access to articles.

$588

$360/year

billed annually
Start Free Trial

14-day Free Trial