Access the full text.
Sign up today, get DeepDyve free for 14 days.
M. Magidin, J. Pittman, K. Hirschi, B. Bartel (2003)
ILR2, a novel gene regulating IAA conjugate sensitivity and metal transport in Arabidopsis thaliana.The Plant journal : for cell and molecular biology, 35 4
Z. Wu, F. Liang, B. Hong, J.C. Young, M.R. Sussman, J.F. Harper, H. Sze (2002)
An ER-bound Ca2+/Mn2+ pump, ECA1, supports plant growth and confers tolerance to Mn2+ stressPlant Physiol., 130
N. Cheng, J. Pittman, B. Barkla, T. Shigaki, K. Hirschi (2003)
The Arabidopsis cax1 Mutant Exhibits Impaired Ion Homeostasis, Development, and Hormonal Responses and Reveals Interplay among Vacuolar Transporters Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.007385.The Plant Cell Online, 15
K. Hirschi (1999)
Expression of Arabidopsis CAX1 in Tobacco: Altered Calcium Homeostasis and Increased Stress SensitivityPlant Cell, 11
E. Delhaize, T. Kataoka, D. Hebb, R. White, P. Ryan (2003)
Genes Encoding Proteins of the Cation Diffusion Facilitator Family That Confer Manganese Tolerance Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.009134.The Plant Cell Online, 15
N. Cheng, J. Pittman, T. Shigaki, K. Hirschi (2002)
Characterization of CAX4, an Arabidopsis H+/Cation Antiporter1Plant Physiology, 128
K. Hirschi (2001)
Vacuolar H+/Ca2+ transport: who's directing the traffic?Trends in plant science, 6 3
Robin Graham, R. Hannam, N. Uren (1988)
Manganese in Soils and Plants
L. Williams, S. Nelson, J. Hall (1990)
Characterization of solute transport in plasma membrane vesicles isolated from cotyledons ofRicinus communis L.Planta, 182
S. Clough, A. Bent (1998)
Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.The Plant journal : for cell and molecular biology, 16 6
J. Pittman, K. Hirschi (2001)
Regulation of CAX1, an Arabidopsis Ca(2+)/H+ antiporter. Identification of an N-terminal autoinhibitory domain.Plant physiology, 127 3
P. Krysan, Jeff Young, F. Tax, M. Sussman (1996)
Identification of transferred DNA insertions within Arabidopsis genes involved in signal transduction and ion transport.Proceedings of the National Academy of Sciences of the United States of America, 93 15
J. Loneragan (1988)
Distribution and Movement of Manganese in Plants, 33
O. Shaul, D. Hilgemann, Janice de‐Almeida‐Engler, M. Montagu, D. Inzé, G. Galili (1999)
Cloning and characterization of a novel Mg2+/H+ exchangerThe EMBO Journal, 18
Z. Rengel (2000)
Manganese uptake and transport in plants.Metal ions in biological systems, 37
J. Pittman, C. Sreevidya, T. Shigaki, H. Ueoka‐Nakanishi, K. Hirschi (2002)
Distinct N-Terminal Regulatory Domains of Ca2+/H+ Antiporters1Plant Physiology, 130
T. Shigaki, J. Pittman, K. Hirschi (2003)
Manganese Specificity Determinants in the ArabidopsisMetal/H+ Antiporter CAX2*The Journal of Biological Chemistry, 278
Gabriel Schaaf, E. Catoni, M. Fitz, R. Schwacke, A. Schneider, N. Wirén, W. Frommer (2002)
A Putative Role for the Vacuolar Calcium/Manganese Proton Antiporter AtCAX2 in Heavy Metal DetoxificationPlant Biology, 4
N. Cheng, J. Pittman, Jian‐Kang Zhu, K. Hirschi (2004)
The Protein Kinase SOS2 Activates the Arabidopsis H+/Ca2+ Antiporter CAX1 to Integrate Calcium Transport and Salt Tolerance*Journal of Biological Chemistry, 279
A. Gonzalez, Victor Korenkov, G. Wagner (1999)
A comparison of Zn, Mn, Cd, and Ca transport mechanisms in oat root tonoplast vesiclesPhysiologia Plantarum, 106
Tsuyoshi Ohnishi, Robert Gall, Michael Mayer (1975)
An improved assay of inorganic phosphate in the presence of extralabile phosphate compounds: application to the ATPase assay in the presence of phosphocreatine.Analytical biochemistry, 69 1
Kelly Curtis, P. Kane (2002)
Novel Vacuolar H+-ATPase Complexes Resulting from Overproduction of Vma5p and Vma13p*The Journal of Biological Chemistry, 277
E. Connolly, J. Fett, M. Guerinot (2002)
Expression of the IRT1 Metal Transporter Is Controlled by Metals at the Levels of Transcript and Protein Accumulation Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.001263.The Plant Cell Online, 14
G. Vert, N. Grotz, F. Dédaldéchamp, F. Gaymard, M. Guerinot, J. Briat, C. Curie (2002)
IRT1, an Arabidopsis Transporter Essential for Iron Uptake from the Soil and for Plant Growth Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.001388.The Plant Cell Online, 14
E. Delhaize, T. Kataoka, D.M. Hebb, R.G. White, P.R. Ryan (2003)
Genes encoding proteins of the cation diffusion facilitator family that confer manganese tolerancePlant Cell, 15
J. Alonso, A. Stepanova, Thomas Leisse, Christopher Kim, Huaming Chen, P. Shinn, Denise Stevenson, J. Zimmerman, Pascual Barajas, Rosa Cheuk, Carmelita Gadrinab, Collen Heller, Albert Jeske, Eric Koesema, Cristina Meyers, Holly Parker, Lance Prednis, Yasser Ansari, Nathan Choy, Hashim Deen, M. Geralt, N. Hazari, Emily Hom, Meagan Karnes, C. Mulholland, Ral Ndubaku, Ian Schmidt, P. Guzmán, Laura Aguilar-Henonin, M. Schmid, D. Weigel, D. Carter, T. Marchand, E. Risseeuw, Debra Brogden, Albana Zeko, W. Crosby, C. Berry, J. Ecker (2003)
Genome-Wide Insertional Mutagenesis of Arabidopsis thalianaScience, 301
A. Hull, Rekha Vij, J. Celenza (2000)
Arabidopsis cytochrome P450s that catalyze the first step of tryptophan-dependent indole-3-acetic acid biosynthesis.Proceedings of the National Academy of Sciences of the United States of America, 97 5
K. Hirschi (2003)
Insertional mutants: a foundation for assessing gene function.Trends in plant science, 8 5
S. Blackford, P. Rea, Dale Sanders (1990)
Voltage sensitivity of H+/Ca2+ antiport in higher plant tonoplast suggests a role in vacuolar calcium accumulation.The Journal of biological chemistry, 265 17
S. Blackford (1990)
9617J. Biol. Chem., 265
P. Mäser, S. Thomine, J. Schroeder, J. Ward, K. Hirschi, H. Sze, Ina Talke, A. Amtmann, F. Maathuis, D. Sanders, J. Harper, J. Tchieu, M. Gribskov, M. Persans, D. Salt, Sun Kim, M. Guerinot (2001)
Phylogenetic relationships within cation transporter families of Arabidopsis.Plant physiology, 126 4
Zhong-Yi Wu, F. Liang, B. Hong, J. Young, M. Sussman, J. Harper, H. Sze (2002)
An Endoplasmic Reticulum-Bound Ca2+/Mn2+Pump, ECA1, Supports Plant Growth and Confers Tolerance to Mn2+ Stress1Plant Physiology, 130
Anthony Ho, D. Ward, J. Kaplan (2002)
Transition metal transport in yeast.Annual review of microbiology, 56
K. Cunningham, G. Fink (1996)
Calcineurin inhibits VCX1-dependent H+/Ca2+ exchange and induces Ca2+ ATPases in Saccharomyces cerevisiaeMolecular and Cellular Biology, 16
G. Vert, N. Grotz, F. Dédaldéchamp, F. Gaymard, M.L. Guerinot, J.-F. Brait, C. Curie (2002)
IRT1, an Arabidopsis transporter essential for iron uptake from the soil and for plant growthPlant Cell, 14
M. Minet, M. Dufour, F. Lacroute (1992)
Complementation of Saccharomyces cerevisiae auxotrophic mutants by Arabidopsis thaliana cDNAs.The Plant journal : for cell and molecular biology, 2 3
P. Mäser, S. Thomine, J.I. Schroeder, J.M. Ward, K. Hirschi, H. Sze, I.N. Talke, A. Amtmann, F.J.M. Maathuis, D. Sanders, J.F. Harper, J. Tchieu, M. Gribskov, M.W. Persans, D.E. Salt, S.A. Kim, M.L. Guerinot (2001)
Phylogenetic relationships within cation-transporter families of Arabidopsis thalianaPlant Physiol., 126
H. Sze, F. Liang, Ildoo Hwang, A. Curran, J. Harper (2000)
Diversity and regulation of plant Ca2+ pumps: insights from expression in yeast.Annual review of plant physiology and plant molecular biology, 51
K. Hirschi, Victor Korenkov, Nathaniel Wilganowski, G. Wagner (2000)
Expression of arabidopsis CAX2 in tobacco. Altered metal accumulation and increased manganese tolerance.Plant physiology, 124 1
E.L. Connolly, J.P. Fett, M.L. Guerinot (2002)
Expression of the IRT1 metal transporter is controlled by metals at the levels of transcript and protein accumulationPlant Cell, 14
F. Maathuis, V. Filatov, P. Herzyk, G. Krijger, K. Axelsen, Sixue Chen, B. Green, Yi Li, K. Madagan, R. Sánchez-Fernández, B. Forde, M. Palmgren, P. Rea, L. Williams, D. Sanders, A. Amtmann (2003)
Transcriptome analysis of root transporters reveals participation of multiple gene families in the response to cation stress.The Plant journal : for cell and molecular biology, 35 6
R. Gaxiola, G. Fink, K. Hirschi (2002)
Genetic Manipulation of Vacuolar Proton Pumps and Transporters1,2Plant Physiology, 129
Gundula Gries, G. Wagner (1998)
Association of nickel versus transport of cadmium and calcium in tonoplast vesicles of oat rootsPlanta, 204
K. Hirschi, R. Zhen, K. Cunningham, P. Rea, G. Fink (1996)
CAX1, an H+/Ca2+ antiporter from Arabidopsis.Proceedings of the National Academy of Sciences of the United States of America, 93 16
Z. Rengel (2000)
Metal Ions in Biological Systems
S. Thomine, F. Lelièvre, E. Debarbieux, J. Schroeder, H. Barbier-Brygoo (2003)
AtNRAMP3, a multispecific vacuolar metal transporter involved in plant responses to iron deficiency.The Plant journal : for cell and molecular biology, 34 5
H. Marschner (1988)
Mineral Nutrition of Higher Plants
H. Shi, F.J. Quintero, J.M. Pardo, J.-K. Zhu (2002)
The putative plasma membrane Na+/H+ antiporter SOS1 controls long-distance Na+ transport in plantsPlant Cell, 14
Takehiro Kamiya, M. Maeshima (2004)
Residues in Internal Repeats of the Rice Cation/H+ Exchanger Are Involved in the Transport and Selection of Cations*Journal of Biological Chemistry, 279
J. Pittman, T. Shigaki, N. Cheng, K. Hirschi (2002)
Mechanism of N-terminal Autoinhibition in theArabidopsis Ca2+/H+ Antiporter CAX1*The Journal of Biological Chemistry, 277
D. Salt, G. Wagner (1993)
Cadmium transport across tonoplast of vesicles from oat roots. Evidence for a Cd2+/H+ antiport activity.The Journal of biological chemistry, 268 17
J. Pittman, K. Hirschi (2003)
Don't shoot the (second) messenger: endomembrane transporters and binding proteins modulate cytosolic Ca2+ levels.Current opinion in plant biology, 6 3
Huazhong Shi, F. Quintero, J. Pardo, Jian‐Kang Zhu (2002)
The Putative Plasma Membrane Na+/H+ Antiporter SOS1 Controls Long-Distance Na+ Transport in Plants Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010371.The Plant Cell Online, 14
L.E. Williams, S.J. Nelson, J.L. Hall (1990)
Characterisation of solute transport in plasma membrane vesicles isolated from cotyledons of Ricinus communis I. ATPase and pyrophosphatase activities associated with a plasma membrane fraction isolated by phase partitioningPlanta, 182
D. Radisky, J. Kaplan (1999)
Regulation of Transition Metal Transport across the Yeast Plasma Membrane*The Journal of Biological Chemistry, 274
N.-H. Cheng, J.K. Pittman, B.J. Barkla, T. Shigaki, K.D. Hirschi (2003)
The Arabidopsis cax1 mutant exhibits impaired ion homeostasis, development, and hormonal responses, and reveals interplay among vacuolar transportersPlant Cell, 15
C. MacDiarmid, M. Milanick, D. Eide (2002)
Biochemical Properties of Vacuolar Zinc Transport Systems ofSaccharomyces cerevisiae *The Journal of Biological Chemistry, 277
H. Sze, K. Schumacher, Mathias Müller, S. Padmanaban, L. Taiz (2002)
A simple nomenclature for a complex proton pump: VHA genes encode the vacuolar H(+)-ATPase.Trends in plant science, 7 4
The vacuolar sequestration of metals is an important metal tolerance mechanism in plants. The Arabidopsis thaliana vacuolar transporters CAX1 and CAX2 were originally identified in a Saccharomyces cerevisiae suppression screen as Ca2+/H+ antiporters. CAX2 has a low affinity for Ca2+ but can transport other metals including Mn2+ and Cd2+. Here we demonstrate that unlike cax1 mutants, CAX2 insertional mutants caused no discernable morphological phenotypes or alterations in Ca2+/H+ antiport activity. However, cax2 lines exhibited a reduction in vacuolar Mn2+/H+ antiport and, like cax1 mutants, reduced V-type H+-ATPase (V-ATPase) activity. Analysis of a CAX2 promoter β-glucoronidase (GUS) reporter gene fusion confirmed that CAX2 was expressed throughout the plant and strongly expressed in flower tissue, vascular tissue and in the apical meristem of young plants. Heterologous expression in yeast identified an N-terminal regulatory region in CAX2, suggesting that Arabidopsis contains multiple cation/H+ antiporters with shared regulatory features. Furthermore, despite significant variations in morphological and biochemical phenotypes, cax1 and cax2 lines both significantly alter V-ATPase activity, hinting at coordinate regulation among transporters driven by H+ gradients and the V-ATPase.
Plant Molecular Biology – Springer Journals
Published: Apr 7, 2005
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.