Function of the evolutionarily conserved plant methionine-S-sulfoxide reductase without thecatalytic residue

Function of the evolutionarily conserved plant methionine-S-sulfoxide reductase without... In plants, two types of methionine sulfoxide reductase (MSR) exist, namely methionine-S-sulfoxide reductase (MSRA) and methionine-R-sulfoxide reductase (MSRB). These enzymes catalyze the reduction of methionine sulfoxides (MetO) back to methionine (Met) by a catalytic cysteine (Cys) and one or two resolving Cys residues. Interestingly, a group of MSRA encoded by plant genomes does not have a catalytic residue. We asked that if this group of MSRA did not have any function (as fitness), why it was not lost during the evolutionary process. To challenge this question, we analyzed the gene family encoding MSRA in soybean (GmMSRAs). We found seven genes encoding GmMSRAs, which included three segmental duplicated pairs. Among them, a pair of duplicated genes, namely GmMSRA1 and GmMSRA6, was without a catalytic Cys residue. Pseudogenes were ruled out as their transcripts were detected in various tissues and their Ka/Ks ratio indicated a negative selection pressure. In vivo analysis in Δ3MSR yeast strain indicated that the GmMSRA6 did not have activity toward MetO, contrasting to GmMSRA3 which had catalytic Cys and had activity. When exposed to H O -induced oxidative stress, GmMSRA6 did not confer any 2 2 protection to the Δ3MSR yeast strain. Overexpression of GmMSRA6 in Arabidopsis http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Protoplasma Springer Journals

Function of the evolutionarily conserved plant methionine-S-sulfoxide reductase without thecatalytic residue

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Publisher
Springer Journals
Copyright
Copyright © 2018 by Springer-Verlag GmbH Austria, part of Springer Nature
Subject
Life Sciences; Cell Biology; Plant Sciences; Zoology
ISSN
0033-183X
eISSN
1615-6102
D.O.I.
10.1007/s00709-018-1266-5
Publisher site
See Article on Publisher Site

Abstract

In plants, two types of methionine sulfoxide reductase (MSR) exist, namely methionine-S-sulfoxide reductase (MSRA) and methionine-R-sulfoxide reductase (MSRB). These enzymes catalyze the reduction of methionine sulfoxides (MetO) back to methionine (Met) by a catalytic cysteine (Cys) and one or two resolving Cys residues. Interestingly, a group of MSRA encoded by plant genomes does not have a catalytic residue. We asked that if this group of MSRA did not have any function (as fitness), why it was not lost during the evolutionary process. To challenge this question, we analyzed the gene family encoding MSRA in soybean (GmMSRAs). We found seven genes encoding GmMSRAs, which included three segmental duplicated pairs. Among them, a pair of duplicated genes, namely GmMSRA1 and GmMSRA6, was without a catalytic Cys residue. Pseudogenes were ruled out as their transcripts were detected in various tissues and their Ka/Ks ratio indicated a negative selection pressure. In vivo analysis in Δ3MSR yeast strain indicated that the GmMSRA6 did not have activity toward MetO, contrasting to GmMSRA3 which had catalytic Cys and had activity. When exposed to H O -induced oxidative stress, GmMSRA6 did not confer any 2 2 protection to the Δ3MSR yeast strain. Overexpression of GmMSRA6 in Arabidopsis

Journal

ProtoplasmaSpringer Journals

Published: May 28, 2018

References

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