1070-4272/03/7605-0818$25.00C2003 MAIK [Nauka/Interperiodica]
Russian Journal of Applied Chemistry, Vol. 76, No. 5, 2003, pp. 818!821. Translated from Zhurnal Prikladnoi Khimii, Vol. 76, No. 5,
2003, pp. 847!850.
Original Russian Text Copyright + 2003 by Kopeikin, Valueva, Kipper, Filippov, Khlebosolova, Borovikova, Lavrent’ev.
AND POLYMERIC MATERIALS
Formation of Se
Nanoparticles in an Aqueous
V. V. Kopeikin, S. V. Valueva, A. I. Kipper, A. P. Filippov, E. N. Khlebosolova,
L. N. Borovikova, and V. K. Lavrent’ev
Institute of Macromolecular Compounds, Russian Academy of Sciences, St. Petersburg, Russia
Received December 23, 2002
Abstract-The nanostructures formed by reduction of Se(IV) in the selenite!ascorbate redox system in an
aqueous solution of supermacromolecular polycation, poly[trimethyl(methacryloyloxyethyl)ammonium]
methyl sulfate, were studied by static and dynamic optical scattering and flow birefringence.
Formation of uncharged metal nanoparticles of
13100 nm size, which have energy-saturated surface
and exhibit certain anomalies in physicochemical
properties, is extensively studied .
However, such metal nanoparticles are unstable in
aqueous solutions in the absence of stabilizing agents.
One of the most promising procedures for preparing
stable metal nanoparticles is reduction of their ionic
species in polymer solutions. In the course of the
pseudo-template synthesis, macromolecules and form-
ing nanoparticles recognize each other, which allows
control of the nanoparticle size by varying the struc-
ture and molecular weight of the polymer .
Such polymer-stabilized nanoparticles are of par-
ticular importance as highly selective catalytic sys-
tems and adsorption matrices for immunoassay, and,
due to the rapid development of the biological nano-
technologies, as active components of drugs and trans-
port systems for biologically active compounds .
However, much less attention was given to non-
metal nanoparticles, especially those of amorphous
), which, in contrast to metal
nanoparticles, can be described as an inorganic poly-
mer containing predominantly the Se
fragments bound by the Se3Se covalent bonds .
exhibits unique photoelectric and semi-
conductor properties and X-ray sensitivity; it is used
as highly sensitive biological sensor for immunoassay
 and chromatographically mobile affine reagent .
Moreover, selenium is an imperative element for
normal vital activity, it exhibits antioxidantive, anti-
mutagenic, immunnostimulating, and detoxication
activity . Therefore, selenium and its compounds
are of particular interest as potential drugs.
As shown in , the reduction of sodium selenite
with glutathione in aqueous solution of bovine serum
albumin (BSA) yields red nano-a-Se
, which is weak-
ly toxic but retains the biologically active properties
typical for ionic selenium species. It was found that
particles are predominantly stabilized
by adsorption processes, in which the noncovalent
(poorly understood at present) interactions of the BSA
macromolecules with nanoparticles play the decisive
To reveal the nature of these interactions and their
contribution to stabilization of nano-a-Se
, we studied
formation of selenium(0) nanoparticles in the presence
of a synthetic polybase and analyzed the morphologi-
cal characteristics of the resulting adsorbates by vari-
ous optical methods.
In our work we studied nanoparticles of a-Se
pared by reduction of selenious acid (SA) with ascor-
bic acid in the presence of a polymer and without it.
The reagent purity was no less than 99.99%. The
reduction kinetics was studied at the SA concentration
of 1.7 mM at 20oC on a Specord M40 spectrophotom-
eter (l 320 nm).
As a polymeric stabilizer we used supermacromolec-
methyl sulfate [polybase (PB)] with M
intrinsic viscosity in 1 M NaNO
= 4.5 dl g