Fluorescent in situ mapping of the murine deleted in split hand/split foot 1 (dss1) gene to Chromosome 6

Fluorescent in situ mapping of the murine deleted in split hand/split foot 1 (dss1) gene to... 704 Mammalian Genome 8, Brief Data Reports COL1A2-PON1-PON3-PON2-DSS 1-DLX6-DLX5-ASNS- Fluorescent in situ mapping of the murine (ACHE-EPO-PCOLCE-GNB2)-CUTL1-7q22.1. calcr, cola2, deleted in split hand/split foot 1 (dssl) gene ponl, pon3, pon2, and now dssl map to mouse Chr 6, and asns, to Chromosome 6 and cutll map to mouse Chr 5. Therefore, ache, epo, pcolce, gnb2, the region where synteny falls off is between DSS 1 and ASNS, which are separated by approximately 1.5 Mb in human [6]. At the Michael A. Crackower, l'z Henny H.Q. Heng, 2 present time the only genes mapping to this region are DLX5 and Xiaomei Shi, 2 Stephen W. Scherer, 2 Lap-Chee Tsui 1'2 DLX6; however, their map position in mouse has not been re- ported. These genes also may play a role in SHFM1 [1]. It would IDepartment of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario, Canada be useful to establish the chromosomal positions of these genes in 2Department of Genetics, The Hospital for Sick Children, 555 mouse to refine the boundaries of synteny as well as for evaluating University Avenue, Toronto, Ontario M5G IX8, Canada potential mouse models for SHFM1. Received: 7 February 1997 / Accepted: 26 April 1997 Acknowledgments: This work is supported by grants from the Canadian Genetic Disease Network, the Howard Hughes Medical Institute (Interna- Species: Mouse tional Scholar Program), and the Canadian Genome Analysis and Tech- Locus name: deleted in split hand/split foot 1 nology Program to L.-C. Tsui. M.A. Crackower is supported by an MRC Locus symbol: dssl studentship. L.-C. Tsui is an MRC Senior Scientist. Map position: 6A Method of mapping: Fluorescence in situ hybridization (FISH) References Database deposit information: Genbank Accession # U41626 1. Crackower MA, Scherer SW, Romens JM, Hui C-C, Poorkaj P, Soder Molecular reagents used for mapping: A mouse phage clone was S, Cobben JM, Hudgins L, Evans JP, Tsui L-C (1996) Hum Mol Genet 5,571-579 isolated from a 129 total genomic library with a full-length dssl 2. Heng HHQ, Squire J, Tsui L-C (1992) Proc Natl Acad Sci USA 89, cDNA [1] as probe. The phage clone was biotinylated with dATP 9509-9513 and the BRL Bionick labeling kit [2], and used in FISH analysis 3. Heng HHQ, Tsui L-C (1993) Chromosoma 102, 325-332 according to previously described methods [2,3]. 4. Crackower MA, Scherer SW, Heng HHQ, Tsui L-C (1997) Mamm Previously idenafied homologs: Human DSS1 maps to 7@1.3- Genome 8, 159-160 q22.1 [1], and a human pseudogene DSS1P1 [4] has been mapped 5. Grzeschik K-H, Tsui L-C, Green ED (1993) Cytogenet Cell Genet 65, to Chromosome (Chr) 5q14 by FISH. 51-73 Discussion: DSS1 was isolated as a candidate gene for split hand/ 6. Scherer SW, Poorkaj P, Massa H, Soder S, Alien T, Nunes M, Geshuri split foot malformation (SHFM1), which maps to human Chr D, Wong E, Belloni E, Little S, Zhou L, Becker D, Kere J, Iguatins J, 7q21.3-q22.1 [ 1 ]. The gene is highly conserved through evolution, Niikawa N, Fukushima Y, Hasegawa T, Weissenbach J, Boncinelli E, and the mouse and human genes are 100% identical at the amino Trask B, Tsui L-C, Evans JP (1994) Hum Mol Genet 3, 1345-1354 acid level, but the putative gene product has no significant homol- ogy to any known protein or protein motifs [I]. RNA in situ hybridization studies indicate that the gene is widely expressed New polymorphism and linkage mapping of during embryogenesis in the limb buds, branchial arches, skin, the bovine lactotransferrin gene genital bud, and other tissues in which epithelial mesenchymal inductive interactions are critical for proper development [1]. Al- though a definitive biological role for this gene has not yet been I. Martin-Burriel, 1 R. Osta, 1 W. Barendse, 2 determined, a mouse knockout model is being generated to address P. Zaragoza 1 this question. Genes localized to human 7q21.3 and human 7q22.1 map to 1Laboratorio de Genrtica Bioquimica, Facultad de Veterinaria, mouse Chr. 6 and 5 respectively [5], but the precise site of diver- Universidad de Zaragoza, Miguel Servet, 177. 50013 Zaragoza, Spain zCSIRO, Division of Tropical Animal Production, Level 3, Gehrmann gence is unknown. The gene order in human is: 7q21.3-CALCR- Laboratories, Research Road, University of Queensland-St. Lucia, Brisbane, 4072, Australia Correspondence to: L-C. Tsui Received: 10 April 1997 / Accepted: 11 April 1997 Species: Bovine Locus name: Lactotransferrin Locus symbol: LTF Map position: LTF is located on bovine Chr 22 linked to GPX1 [1], showing no recombinants and a lod score of 11.44. Method of mapping: A total of 223 animals representing 11 in- formative families of the International Bovine Reference Panel (IBRP) were genotyped for linkage analysis. Genotypes were merged with the cattle Genotypic Database, and all possible pair- waise comparisons were performed with CRI-MAP (v2.4 SunOs). GenBank accession number: L19987 [2] PCR primer sequences: Forward: 5'-GATTCAAGCTGAG- GCATrCC-3'; Reverse: 5'-CCAAAGTGGCCAATTTGAC-3'. Method of detection: The sequence for bovine LTF shows an A17 tract in intron 6. Primers for the PCR were designed on the basis Fig. 1. Diagram showing FISH mapping results with a dssl-positive 129 of the flanking sequences. Amplified fragment showed the ex- phage probe. Among 100 mitotic figures checked, 75 showed double sig- pected size (164 bp), and PCR products showed length polymor- nals on Chr 6. The precise localization was determined from the average of 10 photographs. Each dot represents double FISH signals detected on mouse Chr 6. Correspondence to: I. Martfn-Burriel http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Mammalian Genome Springer Journals

Fluorescent in situ mapping of the murine deleted in split hand/split foot 1 (dss1) gene to Chromosome 6

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Copyright © 1997 by Springer-Verlag
Life Sciences; Cell Biology; Anatomy; Zoology
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