Fine characterization of the antigenic site within the flap region in the protease protein of HIV-1

Fine characterization of the antigenic site within the flap region in the protease protein of HIV-1 The aspartate protease encoded by human immunodeficiency virus type 1 is essential for cleavage of the gag and gag-pol precursor proteins. The majority of HIV-1-antibody-positive sera react with the protease. In this study we used a substitution set of peptides for detailed characterization of the earlierdefined antigenic site (aa 44–58) within the central “flap”region, also important in the context of conformationalflexibility during protease inhibitor binding. We found thatisoleucine at position 54 was important for creating anantigenic site required for binding of anti-HIV-1 sera. The identification of structurally essential amino acids in theflap region of HIV-1 PR may have important implications infuture development of antiviral drugs. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of Virology Springer Journals

Fine characterization of the antigenic site within the flap region in the protease protein of HIV-1

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Publisher
Springer-Verlag
Copyright
Copyright © 2000 by Springer-Verlag/Wien
Subject
Legacy
ISSN
0304-8608
eISSN
1432-8798
D.O.I.
10.1007/s007050050028
Publisher site
See Article on Publisher Site

Abstract

The aspartate protease encoded by human immunodeficiency virus type 1 is essential for cleavage of the gag and gag-pol precursor proteins. The majority of HIV-1-antibody-positive sera react with the protease. In this study we used a substitution set of peptides for detailed characterization of the earlierdefined antigenic site (aa 44–58) within the central “flap”region, also important in the context of conformationalflexibility during protease inhibitor binding. We found thatisoleucine at position 54 was important for creating anantigenic site required for binding of anti-HIV-1 sera. The identification of structurally essential amino acids in theflap region of HIV-1 PR may have important implications infuture development of antiviral drugs.

Journal

Archives of VirologySpringer Journals

Published: Feb 1, 2000

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