We studied the influence of the duration of the joint incubation of cattle oocytes and spermatozoa (18 versus 1 h) on fertilization, cleavage, and embryonic development in vitrountil the blastocyst stage. Spermatozoa of a bull of the Britanofrizskaya breed were used in the experiments. It was shown in the first experimental series that after a long-term incubation with the spermatozoa, the percentage of penetrated eggs increased 71.7 and 56.0% (p< 0.05) after 18-hour and 1-hour incubation, respectively. However, no differences were found in the number of normally fertilized eggs: 46.5 and 39.0%, respectively. In the second experimental series, no significant differences were found in either the number of cleaving embryos (41.2 and 32.2%, respectively) or the capacity of cleaving embryos to develop in vitrountil the blastocyst stage (21.7 and 15.8%, respectively). Thus, reduction in the time of the joint incubation of cattle gametes upon in vitrofertilization to 1 h did not reduce the number of normally fertilized eggs and did not affect their capacity for subsequent in vitrodevelopment.
Russian Journal of Developmental Biology – Springer Journals
Published: Oct 9, 2004
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