ISSN 1021-4437, Russian Journal of Plant Physiology, 2008, Vol. 55, No. 5, pp. 694–698. © Pleiades Publishing, Ltd., 2008.
Original Russian Text © M.Z. Liang, L.J. Ou, L.X. Deng, S. Luan, L.B. Chen, 2008, published in Fiziologiya Rastenii, 2008, Vol. 55, No. 5, pp. 771–776.
Male fertility of photoperiod- and thermosensitive
genic male sterile rice (PMS) is inﬂuenced by photope-
riod and temperature alterations under environmental
conditions . That makes it a desirable germplasm for
the development of “two-line” hybrids in rice-breeding
programs. These plants can be used not only as male-
sterile lines but also as maintainer lines to produce
hybrid rice seeds through a simple, less expensive, and
more efﬁcient “two-line” hybrid seed production sys-
tem . Photoperiod varies gradually and periodically
throughout the year based on the latitude and solar
intensity and remains relatively stable over period of
years ; however, temperature widely varies during
hybrid seed production, being unstable. This probably
poses a great risk while producing hybrid rice from
PMS rice line. Therefore, breeding of a PMS line with
stable sterility is preferred by most rice breeders.
Since the appearance of the mutant Nongken 58S
rice strain from late season japonica rice Nongken 58
, many rice strains sensitive to photoperiod and tem-
perature have been developed for their application to
the “two-line” hybrid rice system [5–8]. However, the
most commonly applied are
rice strains. The
sterility of these
rice strains is also inﬂuenced
This text was submitted by the authors in English.
by temperature because the critical photoperiod for
inducing male sterility in rice exceeds 14 h [3–9]. We
PMS rice with a shorter critical pho-
toperiod and stable sterility under long-day-length con-
ditions. In the current report, we present data on the fer-
tility response to photoperiod and temperature in the
PMS rice line Changguang S.
MATERIALS AND METHODS
Changguang S is a rice strain developed by cross-
breeding temperature-sensitive male serile (TMS) rice
Shuangdi pei’ai  and PMS rice Nongken 58S. As
the controls, we used two typical PMS rice strains,
namely, Nongken 58S and 7001S, and two typical TMS
rice strains, namely, 810S and Zhu 1S and P(T) MS rice
strain Pei’ai 64S.
The experiment was conducted at the Hunan Nor-
mal University Farm in Chungsha, Hunan Province,
E) during the rice-growing
season (April–October) in 2005 and repeated in 2006.
The seedlings were raised in a ﬁeld, being sowed ten
times with 10-day intervals beginning from March 30.
Five-leaf stage seedlings were individually trans-
planted into the ﬁeld at a hill spacing of
transplanted 5 plants of each sowing date into plastic
pots that were 20 cm in diameter; 10 pots were planted
for each treatment. All plants were grown under natural
long-day-length and high-temperature conditions until
Fertility Response to Photoperiod and Temperature in Indica
Photoperiod-Sensitive Male Sterile Rice
M. Z. Liang, L. J. Ou, L. X. Deng, S. Luan, and L. B. Chen
College of Life Science, Hunan Normal University, Changsha, 410081, China;
Received: August 2, 2007
—The aim of this work was to develop a photoperiod-sensitive male sterile rice with stable sterility.
We developed Changguang S, an indica rice strain, by using a short critical day length. Differences in the fer-
tility responses of Changguang S strain pollen to temperature and photoperiod under natural and controlled con-
ditions were studied. The results showed that Changguang S strain exhibited stable sterility under long-day and
low-temperature conditions (22
C, 15 days). The stability of sterility was signiﬁcantly higher than that of other
such rice strains, Nongken 58S and 7001S. The critical photoperiod for inducing male sterility in Changguang S was
13 h or shorter, and its duration was signiﬁcantly shorter than that required for rice strains Nongken 58S and
7001S. It is suggested that Changguang S is a typical photoperiod-sensitive male sterile rice strain with a shorter
critical day length and a lower critical temperature. It is promising to apply this strain to two-line hybrid rice
Key words: Oryza sativa - photoperiod sensitivity male sterility - fertility - photoperiod - temperature - pollen
: PMS-photoperiod-sensitive genic male sterile
rice; TMS-temperature-sensitive genic male sterile rice.