Features of respiration and formation of steroidal glycosides in Dioscorea deltoidea cell suspension culture grown in flasks and bioreactors

Features of respiration and formation of steroidal glycosides in Dioscorea deltoidea cell... An important task is to monitor the physiological state of the cell population when scaling apparatus cultivation of higher plants’ cell cultures to industrial volumes. Investigations were carried out on the culture of Dioscorea deltoidea Wall—strain producer of steroidal glycosides (protodioscin and deltoside)—for which growth characteristics and content of furostanol glycosides were determined during the semirunning growth in bubble bioreactors (20 and 630 L); total rate of oxygen uptake and the portion of participation of alternative oxidase (AO) in the process were measured in the exponential growth phase. The measurements were performed for 3–4 subculture cycles for each apparatus, starting from the second cycle from the moment of inoculation. The values of oxygen mass transfer coefficients (K La, h−1) for bioreactors were determined at modes of mixing and aeration optimal for growing strain. The maximal portion of cyanide resistant respiration was determined in 20-liter bubble bioreactor with a point aerating device (up to 60–65% of total respiration rate). The minimal values of (K La (2.0–2.4 h−1) were obtained for the same apparatus, as well as lower levels of furostanol glycosides (4.2–8.0% of dry cell weight). Apparently, this effect was due to insufficiently uniform distribution of oxygen in the nutrient medium. Minimal activity of AO (within 15–25%) was noted for the system with more intensive mass transfer of oxygen-in 630-liter bubble bioreactor with the annular aerator (K La 7.1–8.0 h−1). Higher biosynthetic rates (7.7–13.9% of dry cell weight) were found in the same bioreactor. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Features of respiration and formation of steroidal glycosides in Dioscorea deltoidea cell suspension culture grown in flasks and bioreactors

Loading next page...
 
/lp/springer_journal/features-of-respiration-and-formation-of-steroidal-glycosides-in-KX4o9eECUW
Publisher
Springer Journals
Copyright
Copyright © 2015 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443715040160
Publisher site
See Article on Publisher Site

Abstract

An important task is to monitor the physiological state of the cell population when scaling apparatus cultivation of higher plants’ cell cultures to industrial volumes. Investigations were carried out on the culture of Dioscorea deltoidea Wall—strain producer of steroidal glycosides (protodioscin and deltoside)—for which growth characteristics and content of furostanol glycosides were determined during the semirunning growth in bubble bioreactors (20 and 630 L); total rate of oxygen uptake and the portion of participation of alternative oxidase (AO) in the process were measured in the exponential growth phase. The measurements were performed for 3–4 subculture cycles for each apparatus, starting from the second cycle from the moment of inoculation. The values of oxygen mass transfer coefficients (K La, h−1) for bioreactors were determined at modes of mixing and aeration optimal for growing strain. The maximal portion of cyanide resistant respiration was determined in 20-liter bubble bioreactor with a point aerating device (up to 60–65% of total respiration rate). The minimal values of (K La (2.0–2.4 h−1) were obtained for the same apparatus, as well as lower levels of furostanol glycosides (4.2–8.0% of dry cell weight). Apparently, this effect was due to insufficiently uniform distribution of oxygen in the nutrient medium. Minimal activity of AO (within 15–25%) was noted for the system with more intensive mass transfer of oxygen-in 630-liter bubble bioreactor with the annular aerator (K La 7.1–8.0 h−1). Higher biosynthetic rates (7.7–13.9% of dry cell weight) were found in the same bioreactor.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Jun 17, 2015

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off