Factors influencing T-DNA transferring into pollen of lily in vitro

Factors influencing T-DNA transferring into pollen of lily in vitro Direct pollen transformation method improves the classical transformation procedures because some tissue culture steps and subsequent regeneration can be avoided. A critical step in the development of Agrobacterium-mediated transformation is the establishment of optimum conditions for T-DNA delivery into tissue. The pollen grains of David lily (Lilium davidii Duchartre) are transformable by Agrobacterium during their germination, and extremely high GUS expression frequency of pollen had been achieved (92.7 ± 2.7%), but not for the ungerminated pollen. The culture medium, Agrobacterium cell density, duration of co-cultivation, and the combination of bacterial strains and plasmids should be optimized to get the highest transformation frequency. Thus, a method for pollen monocotyledonous species reproductive tissues transformation by Agrobacterium in monocots has been successfully developed. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Factors influencing T-DNA transferring into pollen of lily in vitro

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Copyright © 2007 by Pleiades Publishing, Ltd.
Life Sciences; Plant Physiology; Plant Sciences
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