Factors determining enzyme activities in soils under Pinus halepensis
and Pinus sylvestris plantations in Spain: a basis for establishing
sustainable forest management strategies
María Belén Turrión
Received: 10 October 2017 / Accepted: 28 February 2018 / Published online: 13 March 2018
INRA and Springer-Verlag France SAS, part of Springer Nature 2018
Key message Water availability and soil pH seem to be major constraints for enzyme activities in calcareous soils under
Pinus halepensis and acidic soils under Pinus sylvestris plantations respectively. Proposals for improving enzyme activities
may include the promotion of broadleaf species to increase soil pH and the modulation of stand density or the imple-
mentation of soil preparation techniques to facilitate water infiltration.
Context Soil enzymes play a key role in nutrient turnover in forest ecosystems, as they are responsible for the transformation of
organic matter into available nutrients for plants. Enzyme activities are commonly influenced by temperature, humidity, nutrient
availability, pH, and organic matter content.
Aims To assess the differences between enzyme activities in calcareous soils below Pinus halepensis and acidic soils below
Pinus sylvestris plantations in Spain and to trace those differences back to edapho-climatic parameters to answer the questions:
Which environmental factors drive enzyme activities in these soils? How can forest management improve them?
Methods The differences in climatic, soil physical, chemical, and biochemical parameters and the correlations between these
parameters and enzyme activities in soils were assessed.
Results Low pH and high level of phenols in acidic soils under Pinus sylvestris and water deficit in calcareous soils under Pinus
halepensis plantations appeared to be the most limiting factors for enzyme activities.
Handling Editor: Erwin Dreyer
Contribution of the co-authors
Teresa Bueis: designed the experiment, carried out the field and
laboratory work, run the data analysis, discussed the results, and wrote
Felipe Bravo: designed the experiment, coordinated the research project,
and corrected the manuscript
Valentín Pando: supported the statistical analysis and corrected the
María-Belén Turrión: designed the experiment, supervised the laboratory
analysis, and corrected the manuscript
Adele Muscolo: supervised the laboratory analysis and corrected the
This article is part of the Topical Collection on Mediterranean pines
* Teresa Bueis
Sustainable Forest Management Research Institute, University of
Valladolid & INIA, Avda. Madrid 44, 34004 Palencia, Spain
Departamento de Ciencias Agroforestales. E.T.S. Ingenierías
Agrarias, Universidad de Valladolid, Palencia, Spain
Departamento de Producción Vegetal y Recursos Forestales. E.T.S.
Ingenierías Agrarias, Universidad de Valladollid, Palencia, Spain
Departamento de Estadística e Investigación Operativa. E.T.S.
Ingenierías Agrarias, Universidad de Valladolid, Palencia, Spain
Dipartimento di Gestione dei Sistemi Agrari e Forestali, Università
degli Studi Mediterranea di Reggio Calabria, Feo di Vito,
89060 Reggio Calabria, Italy
Annals of Forest Science (2018) 75: 34