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LJA Kogelman (2013)
1Front Genet, 4
M Scott, MD Grundy (2012)
Pre-diabetes, metabolic syndrome, and cardiovascular riskJ Am Coll Cardiol, 59
Y Minokoshi (2003)
33609J Biol Chem, 278
S Cirera (2013)
472BMC Res Notes, 6
M Hribal, L Perego, S Lovari, F Andreozzi, R Menghini, C Perego, G Finzi (2003)
Chronic hyperglycemia impairs insulin secretion by affecting insulin receptor expression, splicing, and signaling in RIN beta cell line and human islets of langerhansFASEB J, 17
B Leibiger, I Leibiger, T Moede, S Kemper, R Kulkarni, C Ronald Kahn, L Vargas, P Berggren (2001)
Selective insulin signaling through A and B insulin receptors regulates transcription of insulin and glucokinase genes in pancreatic beta CellsMol Cell, 7
RN Kulkarni, JC Brüning, JN Winnay, C Postic, MA Magnuson, CR Kahn (1999)
Tissue-specific knockout of the insulin receptor in pancreatic Β cells creates an insulin secretory defect similar to that in type 2 diabetesCell, 96
J Oliveira, S Rebuffat, R Gasa, R Gomis (2014)
Targeting type 2 diabetes: lessons from a Knockout model of insulin receptor substrate 2NRP Res Press, 620
C Postic, A Leturque, F Rencurel, R Printz, C Forest, D Granner, J Girard (1993)
The effects of hyperinsulinemia and hyperglycemia on GLUT4 and hexokinase II mRNA and protein in rat skeletal muscle and adipose tissueDiabetes, 42
AE Butler (2003)
102Diabetes, 52
S Cirera (2013)
Highly efficient method for isolation of total RNA from adipose tissueBMC Res Notes, 6
B Leibiger (2001)
559Mol Cell, 7
F Bianchin, R Kaaks, H Vainio (2002)
Overweight, obesity, and cancer riskLancet Oncol, 3
J Whittaker, H Sørensen, V Gadsbøll, J Hinrichsen (2002)
Comparison of the functional insulin binding epitopes of the A and B isoforms of the insulin receptorJ Biol Chem, 277
P Meyts De (2004)
160Novartis Found Symp, 262
K Bouzakri, P Ribaux, T Alejandra, G Parnaud, K Rickenbach, PA Halban (2008)
Rab GTPase-activating protein AS160 is a major downstream effector of protein kinase B/Akt signaling in pancreatic Β-cellsApoptosis, 57
CL Andersen, JL Jensen, TF Ørntoft (2004)
Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization applied to bladder and colon cancer data sets normalization of real-time quantitative reverseCancer Res, 64
I Moltke, N Grarup, M Jørgensen, P Bjerregaard, J Treebak, M Fumagalli, T Korneliussen (2014)
A common greenlandic TBC1D4 variant confers muscle insulin resistance and type 2 diabetesNature
F Bianchin (2002)
565Lancet Oncol, 3
A-B Nygard, C Jørgensen, S Cirera, M Fredholm (2007)
Selection of reference genes for gene expression studies in pig tissues using SYBR green qPCRBMC Mol Biol, 8
CL Andersen (2004)
5245Cancer Res, 64
AE Butler, J Juliette, S Bonner-Weir, R Ritzel, RA Rizza, PC Butler (2003)
Β-cell deficit and increased Β-cell apoptosis in humans with type 2Diabetes, 52
M Imamura, S Maeda (2011)
Genetics of Type 2 diabetes: the GWAS era and future perspectives [review]Endocr J, 58
M Imamura (2011)
723Endocr J, 58
KL Hoehn (2008)
421Cell Metab, 7
LJA Kogelman, HN Kadarmideen, T Mark, P Karlskov-Mortensen, CS Bruun, S Cirera, MJ Jacobsen, CB Jørgensen, M Fredholm (2013)
An F2 pig resource population as a model for genetic studies of obesity and obesity-related diseases in humans: design and genetic parametersFront Genet, 4
M Larance (2005)
37803J Biol Chem, 280
S-X Tan, Y Ng, JG Burchfield, G Ramm, DG Lambright, J Stockli, DE James (2012)
The RabGAP TBC1D4/AS160 Contains an atypical PTB domain that interacts with plasma membrane phospholipids to facilitate GLUT4 trafficking in adipocytesMol Cell Biol
RN Kulkarni (1999)
329Cell, 96
KL Hoehn, C Hohnen-Behrens, A Cederberg, LE Wu, N Turner, T Yuasa, Y Ebina, DE James (2008)
IRS1-independent defects define major nodes of insulin resistanceCell Metab, 7
L Eguez, A Lee, JA Chavez, CP Miinea, S Kane, GE Lienhard, TE McGraw (2005)
Full intracellular retention of GLUT4 requires AS160 Rab GTPase activating proteinCell Metab, 2
M Hribal (2003)
1340FASEB J, 17
P Meyts, J Palsgaard, W Sajid, AM Theede, H Aladdin (2004)
Structural biology of insulin and IGF-1 receptorsNovartis Found Symp, 262
Y Minokoshi, C Ronald Kahn, B Kahn (2003)
Tissue-specific ablation of the GLUT4 glucose transporter or the insulin receptor challenges assumptions about insulin action and glucose homeostasisJ Biol Chem, 278
K Bouzakri (2008)
1195Apoptosis, 57
M Larance, G Ramm, J Stöckli, E Dam, S Winata, V Wasinger, F Simpson (2005)
Characterization of the role of the Rab GTPase-activating protein AS160 in insulin-regulated GLUT4 traffickingJ Biol Chem, 280
L Eguez (2005)
263Cell Metab, 2
Obesity is a world-wide exponentially growing health problem that increases the risk of co-morbidities including metabolic syndrome, pre-diabetes, Type 2 Diabetes Mellitus (T2DM), and cancer. These co-morbidities are all complex conditions constituting a big challenge when searching for susceptibility genes. Identification of relevant genes, which could contribute to an earlier identification of individuals prone to develop diabetes, is urgently needed as many long-term complications can be avoided by preventive measures. Pre-diabetes is mainly associated with hyperglycemia; thus studying this phenotype might provide knowledge on relevant genes implicated in molecular mechanisms underlying pre-diabetes, and contributing to the development of T2DM. In the present study, two groups of pigs with high (HGG, N = 6) and low (NGG, N = 6) fasting plasma glucose level respectively were selected from a large pig population. Transcriptional levels of seven genes involved in the glucose transporter 4 (GLUT4) translocation pathway were studied by quantitative real-time PCR (qPCR) in diabetes relevant tissues (pancreas, adipose tissue, skeletal muscle, liver and kidney). Three of the genes, TBC (Tre-2, BUB2, CDC16) 1 Domain Family Member 4 (TBC1D4), insulin receptor and GLUT4 showed altered expression in some of the tissues. The expression pattern observed is in agreement with what has previously been reported in pre-diabetic humans confirming the pre-diabetic status of our pigs. Moreover, a novel isoform of TBC1D4 was detected by Western blotting using protein extracted from pancreas. The expression level of this novel isoform was further verified by qPCR in all tissues, showing the highest expression in the pancreas.
Mammalian Genome – Springer Journals
Published: Sep 7, 2015
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