Expression pattern analysis of Will Die Slowly genes in Arabidopsis

Expression pattern analysis of Will Die Slowly genes in Arabidopsis Leaf senescence is a developmental programmed cell death (PCD) that occurs as a response to external and internal signals. Several factors, such as the environment, plant hormones, and senescence-associated genes, regulate leaf senescence. In Drosophila melanogaster, Will Die Slowly (WDS) is a WD-repeat protein, which is closely related to PCD. Phylogenetic analysis indicated that eight genes are highly homologous to D. melanogaster WDS (DmWDS) in Columbia ecotype (Col) of Arabidopsis thaliana. In this study, the expression patterns of three close homologues of DmWDS named WDS1, WDS2, and WDS3 were investigated. Real-time PCR revealed the spatio-temporal expression levels of these three genes. No tissue-specific expression of the three AtWDS genes was observed. These genes were expressed at every growth stage; the variation in their expression was similar: the expression of the three AtWDS reached the peak in leaves at the 37 days after sowing, at the time when the first pod initially appeared on the plant and the leaf 7 show 25 to 50% yellow; and the expression of the three AtWDS reached the peak in flowers at the 43.5 days after sowing, at the time when 50% of the flowers bloomed. In addition, the expression level of the three AtWDS peaked at the 48 h after the plants were treated with 0.5 mM salicylic acid (SA). WDS3 also exhibited a high expression at 24, 48, and 72 h. Taken together; these results suggest that AtWDS genes may be involved in plant PCD http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Expression pattern analysis of Will Die Slowly genes in Arabidopsis

Loading next page...
 
/lp/springer_journal/expression-pattern-analysis-of-will-die-slowly-genes-in-arabidopsis-HBzhzJggvH
Publisher
Pleiades Publishing
Copyright
Copyright © 2014 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443714060223
Publisher site
See Article on Publisher Site

Abstract

Leaf senescence is a developmental programmed cell death (PCD) that occurs as a response to external and internal signals. Several factors, such as the environment, plant hormones, and senescence-associated genes, regulate leaf senescence. In Drosophila melanogaster, Will Die Slowly (WDS) is a WD-repeat protein, which is closely related to PCD. Phylogenetic analysis indicated that eight genes are highly homologous to D. melanogaster WDS (DmWDS) in Columbia ecotype (Col) of Arabidopsis thaliana. In this study, the expression patterns of three close homologues of DmWDS named WDS1, WDS2, and WDS3 were investigated. Real-time PCR revealed the spatio-temporal expression levels of these three genes. No tissue-specific expression of the three AtWDS genes was observed. These genes were expressed at every growth stage; the variation in their expression was similar: the expression of the three AtWDS reached the peak in leaves at the 37 days after sowing, at the time when the first pod initially appeared on the plant and the leaf 7 show 25 to 50% yellow; and the expression of the three AtWDS reached the peak in flowers at the 43.5 days after sowing, at the time when 50% of the flowers bloomed. In addition, the expression level of the three AtWDS peaked at the 48 h after the plants were treated with 0.5 mM salicylic acid (SA). WDS3 also exhibited a high expression at 24, 48, and 72 h. Taken together; these results suggest that AtWDS genes may be involved in plant PCD

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 12, 2014

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 12 million articles from more than
10,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Unlimited reading

Read as many articles as you need. Full articles with original layout, charts and figures. Read online, from anywhere.

Stay up to date

Keep up with your field with Personalized Recommendations and Follow Journals to get automatic updates.

Organize your research

It’s easy to organize your research with our built-in tools.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve Freelancer

DeepDyve Pro

Price
FREE
$49/month

$360/year
Save searches from
Google Scholar,
PubMed
Create lists to
organize your research
Export lists, citations
Read DeepDyve articles
Abstract access only
Unlimited access to over
18 million full-text articles
Print
20 pages/month
PDF Discount
20% off