Expression of the Kidney Bean Phenylalanine-Ammonia Lyase Gene in the Hairy Roots of Astragalus sinicus

Expression of the Kidney Bean Phenylalanine-Ammonia Lyase Gene in the Hairy Roots of Astragalus... Using Agrobacterium rhizogenes, Astragalus sinicus plants were transformed with the kidney bean pal5 gene coding for phenylalanine-ammonia lyase (PAL). The hairy root culture thus obtained manifested enhanced PAL activity and lignin content in the cell walls; in addition, the transformed cells differed from the wild-type ones in several electrophysiological indices. In particular, the diffusion component of the total membrane potential of plasmalemma increased in the pal-transformed roots. The authors presume that the volume density of the protein-related negative charge of the cytoplasm increases in the transformed root cells along with changes in the cytoplasmic pH and pCa2+, the extent of coupling of these two indices, and the hydraulic conductivity of plasmodesmata. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Expression of the Kidney Bean Phenylalanine-Ammonia Lyase Gene in the Hairy Roots of Astragalus sinicus

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Publisher
Springer Journals
Copyright
Copyright © 2002 by MAIK “Nauka/Interperiodica”
Subject
Life Sciences; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1023/A:1020973831060
Publisher site
See Article on Publisher Site

Abstract

Using Agrobacterium rhizogenes, Astragalus sinicus plants were transformed with the kidney bean pal5 gene coding for phenylalanine-ammonia lyase (PAL). The hairy root culture thus obtained manifested enhanced PAL activity and lignin content in the cell walls; in addition, the transformed cells differed from the wild-type ones in several electrophysiological indices. In particular, the diffusion component of the total membrane potential of plasmalemma increased in the pal-transformed roots. The authors presume that the volume density of the protein-related negative charge of the cytoplasm increases in the transformed root cells along with changes in the cytoplasmic pH and pCa2+, the extent of coupling of these two indices, and the hydraulic conductivity of plasmodesmata.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Oct 13, 2004

References

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