Expression of SOD and APX genes positively regulates secondary cell wall biosynthesis and promotes plant growth and yield in Arabidopsis under salt stress

Expression of SOD and APX genes positively regulates secondary cell wall biosynthesis and... Abiotic stresses cause accumulation of reactive oxygen species (ROS), such as hydrogen peroxide (H2O2) in plants. Sophisticated mechanisms are required to maintain optimum level of H2O2 that acts as signalling molecule regulating adaptive response to salt stress. CuZn-superoxide dismutase (CuZn-SOD) and ascorbate peroxidase (APX) constitute first line of defence against oxidative stress. In the present study, PaSOD and RaAPX genes from Potentilla atrosanguinea and Rheum australe, respectively were overexpressed individually as well as in combination in Arabidopsis thaliana. Interestingly, PaSOD and dual transgenic lines exhibit enhanced lignin deposition in their vascular bundles with altered S:G ratio under salt stress. RNA-seq analysis revealed that expression of PaSOD gene in single and dual transgenics positively regulates expression of lignin biosynthesis genes and transcription factors (NACs, MYBs, C3Hs and WRKY), leading to enhanced and ectopic deposition of lignin in vascular tissues with larger xylem fibres and alters S:G ratio, as well. In addition, transgenic plants exhibit growth promotion, higher biomass production and increased yield under salt stress as compared to wild type plants. Our results suggest that in dual transgenics, ROS generated during salt stress gets converted into H2O2 by SOD and its optimum level was maintained by APX. This basal level of H2O2 acts as messenger for transcriptional activation of lignin biosynthesis in vascular tissue, which provides mechanical strength to plants. These findings reveal an important role of PaSOD and RaAPX in enhancing salt tolerance of transgenic Arabidopsis via increased accumulation of compatible solutes and by regulating lignin biosynthesis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Plant Molecular Biology Springer Journals

Expression of SOD and APX genes positively regulates secondary cell wall biosynthesis and promotes plant growth and yield in Arabidopsis under salt stress

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Publisher
Springer Netherlands
Copyright
Copyright © 2015 by Springer Science+Business Media Dordrecht
Subject
Life Sciences; Plant Sciences; Biochemistry, general; Plant Pathology
ISSN
0167-4412
eISSN
1573-5028
D.O.I.
10.1007/s11103-015-0301-6
Publisher site
See Article on Publisher Site

References

  • Characterization of digestion residues of alfalfa and orchardgrass leaves by microscopic, spectroscopic and chemical analysis
    Akin, DE; Morrison, WH; Himmelsbach, DS
  • The water–water cycle in chloroplasts: scavenging of active oxygen and dissipation of excess photons
    Asada, K
  • Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis
    Czechowski, T; Stitt, M; Altman, T; Udvardi, MK; Scheible, WR
  • Hydrogen peroxide as a signal controlling plant programmed cell death
    Gechev, TS; Hille, J
  • A parsley 4CL-1 promoter fragment specifies complex expression patterns in transgenic tobacco
    Hauffe, KD; Paszkowski, U; Schulze-Lefert, P; Hahlbrock, K; Dangl, JL; Douglas, CJ
  • Saline stress alters the temporal patterns of xylem differentiation and alternative oxidase expression in developing soybean roots
    Hilal, M; Zenoff, AM; Ponessa, G; Moreno, H; Massa, ED
  • Development of the Casparian strip in primary roots of maize under salt stress
    Karahara, I; Ikeda, A; Kondo, T; Uetake, Y

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