Appl Biochem Biotechnol https://doi.org/10.1007/s12010-018-2773-8 Expression of miRNAs Targeting mTOR and S6K1 Genes of mTOR Signaling Pathway Including miR-96, miR-557, and miR-3182 in Triple-Negative Breast Cancer 1 2,3 Javad Razaviyan & Razie Hadavi & 3 4 Rezvan Tavakoli & Fereshteh Kamani & 1 5,6 Maliheh Paknejad & Samira Mohammadi-Yeganeh Received: 8 February 2018 /Accepted: 23 April 2018 Springer Science+Business Media, LLC, part of Springer Nature 2018 Abstract Triple-negative breast cancer (TNBC) is a highly aggressive form of breast cancer. Aberrant expression of genes in mTOR pathway and their targeting miRNAs plays an important role in TNBC. The aim of this study was to determine the expression of mTOR and S6K1 and their targeting miRNAs in breast cancer cell lines and clinical samples. miRNAs targeting 3′-UTR of mTOR and S6K1 mRNAs were predicted using bioinformatic algorithms. MDA-MB-231, MCF-7, and MCF-10A as well as 20 TNBC samples were analyzed for gene and miRNA expression using quantitative real-time PCR (RT-qPCR). A receiver operating characteristic (ROC) curve analysis was performed for evaluation of candidate miRNAs as diagnostic biomarkers. miR-96 and miR-557 targeting mTOR and S6K1 mRNAs, respectively, were selected, and miR-3182 was selected as the miRNA targeting both genes. The miRNAs were down-regulated in
Applied Biochemistry and Biotechnology – Springer Journals
Published: Jun 4, 2018
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