Expression of ferritin gene in Mesembryanthemum crystallinum plants under different supply with iron and different intensity of oxidative stress

Expression of ferritin gene in Mesembryanthemum crystallinum plants under different supply with... In plants of the facultative halophyte Mesembryanthemum crystallinum L. cultivated under climate-controlled conditions, expression of one of ferritin genes, McFer, the ortholog of arabidopsis AtFer1 gene was studied for the first time. The level of this gene expression occurring in response to oxidative stress and changes in the iron status was similar to that of AtFer1 gene. A dependence of McFer gene expression and ferritin content on the regime of plant supplying with Fe-EDTA on the background of medium salinity (300 mM NaCl), oxidative stress modeling by leaf treatment with paraquat (PQ, 100 μM), or in the presence of antioxidant spermidine (Spd, 1 mM) was analyzed. The level of gene expression was assessed by RT-PCR, whereas the content of ferritin by Western blotting, using the primary polyclonal antibody against pea ferritin. An enhanced production of superoxide radical and hydrogen peroxide at leaf treatment with PQ activated gene expression and ferritin content, whereas ROS scavenging with the antioxidant Spd suppressed gene expression. It is concluded that ferritin deposits in the halophyte M. crystallinum, which we have observed earlier in the chloroplasts of the mesophyll and parenchyma of the vascular system, fulfill not only storage but also protective role by binding the excessive Fe2+, a catalyzer of OH·− production. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Russian Journal of Plant Physiology Springer Journals

Expression of ferritin gene in Mesembryanthemum crystallinum plants under different supply with iron and different intensity of oxidative stress

Loading next page...
 
/lp/springer_journal/expression-of-ferritin-gene-in-mesembryanthemum-crystallinum-plants-kfIOt0Z407
Publisher
SP MAIK Nauka/Interperiodica
Copyright
Copyright © 2011 by Pleiades Publishing, Ltd.
Subject
Life Sciences; Plant Physiology; Plant Sciences
ISSN
1021-4437
eISSN
1608-3407
D.O.I.
10.1134/S1021443711050219
Publisher site
See Article on Publisher Site

Abstract

In plants of the facultative halophyte Mesembryanthemum crystallinum L. cultivated under climate-controlled conditions, expression of one of ferritin genes, McFer, the ortholog of arabidopsis AtFer1 gene was studied for the first time. The level of this gene expression occurring in response to oxidative stress and changes in the iron status was similar to that of AtFer1 gene. A dependence of McFer gene expression and ferritin content on the regime of plant supplying with Fe-EDTA on the background of medium salinity (300 mM NaCl), oxidative stress modeling by leaf treatment with paraquat (PQ, 100 μM), or in the presence of antioxidant spermidine (Spd, 1 mM) was analyzed. The level of gene expression was assessed by RT-PCR, whereas the content of ferritin by Western blotting, using the primary polyclonal antibody against pea ferritin. An enhanced production of superoxide radical and hydrogen peroxide at leaf treatment with PQ activated gene expression and ferritin content, whereas ROS scavenging with the antioxidant Spd suppressed gene expression. It is concluded that ferritin deposits in the halophyte M. crystallinum, which we have observed earlier in the chloroplasts of the mesophyll and parenchyma of the vascular system, fulfill not only storage but also protective role by binding the excessive Fe2+, a catalyzer of OH·− production.

Journal

Russian Journal of Plant PhysiologySpringer Journals

Published: Aug 21, 2011

References

You’re reading a free preview. Subscribe to read the entire article.


DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create lists to
organize your research

Export lists, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off